Anti-Rat CD90 (Thy 1.1) Biotin (Clone OX-7) (mouse IgG1)
DescriptionCedarlane's anti-rat CD90 (Thy 1.1) monoclonal antibody recognizes the Thy 1.1 antigenic determinant on rat as well as mouse cells. This particular determinant has been defined to be monomorphic within rats but polymorphic in the mouse. The OX-7 clone reacts with Thy 1.1 mice (ie. AKR) but not Thy 1.2 mice (ie. CBA, BALB/c). The affinity of the F(ab) fragment of this clone for rat Thy-1.1 is 3 x109 M-1 and for mouse Thy 1.1 is 3 x 108 M-1 (1). The Thy-1.1 antigen is found on a variety of cell types including thymocytes, neuronal cells (mouse, rat) (8), T and immature B cells (rat), breast epithelial cells (rat) (6), and connective tissue (2). OX-7 has been used to determine that the Thy 1.1 molecule is a glycoprotein with 112 amino acids which is homologous to immunoglobulin domains (3).
The Thy-1 antigen is found on a diverse range of cell types (4) and thus it can be used as a cell marker. Furthermore, the binding characteristics of OX-7 have been extensively studied (1) and this appears to be an excellent antibody for studying the killing of Thy-1.1 positive tumour cells with Ab-toxin conjugates (5). In addition, OX-7 can be coupled to Sepharose-4B and used to effectively purify mouse Thy 1.1 and rat Thy-1.1 antigens. This clone has also been reported to work in immunohistochemistry (frozen sections).______________________________________________________________________________________________________Flow Cytometry AnalysisWistar rat thymocytes (left) and splenocytes (right) were stained with anti-CD90 (clone: OX-7) (filled histogram) or mouse IgG1, k isotype control (open histogram).
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