Standard Rabbit Complement, Frozen
Standard Rabbit Complement for use with Human PBLsStandard Rabbit Complement is derived from a pool of normal rabbit serum. It is intended for use as a source of complement for any of the standard lymphocytotoxicity procedures utilizing human peripheral blood lymphocytes (PBL's), including HLA-ABC tissue typing. The Rabbit Complement is added to human PBL's that have been pre-incubated with anti-HLA antisera. The sensitized cells are incubated in the presence of Rabbit Complement for the appropriate length of time, and then scored for degree of killing. Full details of all the standard lymphocytotoxicity techniques are published in the NIAID Manual of Tissue Typing Techniques.