Cedarlane › CL7606F

Anti-Human (CD45R), FITC (clone YTH 80.103), (Rat IgG2b)

Name: Cedarlane
Address: 1210 Turrentine Street Burlington, NC, 27215, Burlington, NC, USA

$364.00 CDN

Description

Cedarlane’s anti-Human CD45R monoclonal antibody specifically binds to human CD45R. This is the longest isoform of the human leucocyte common antigen, CD45. CD45 is a family of single-chain transmembrane glycoproteins with protein phosphatase activity involved in transmembrane signaling. The molecular weight of CD45R is dependent on its level of glycosylation, which varies from its sources of expression: 200 kDa from T cells, 220 kDa from B cells as well as certain activated T cells and other antigen presenting cells. Recognition of antigen may be dependent on expression levels of sialic acid.
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Flow Cytometry Analysis
Human peripheral blood lymphocytes were stained with anti-CD45R (clone: YTH 80.103) (filled histogram) or Rat IgG2b isotype control (open histogram).
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Immunofluorescence Analysis

Purified Anti-Human CD45R (CL7606AP) staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification with DAPI counterstain. (Rat anti-human CD45R (CL7606AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))

References
1) Dianzani U, Redoglia V, Malavasi F, et al. Isoform-specific associations of CD45 with accessory molecules in human T lymphocytes. Eur J Immunol. Feb; 22(2):365-71. 1992.
2) Hale G, Clark M, Waldmann H. Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. May; 134(5):3056-61. 1985.

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