Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b)
DescriptionCedarlane’s purified anti-mouse erythroid cell (Ly-76) mAb is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes.TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive with CL8926AP. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers.This mAb immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western Blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells.This product is suitable for use in flow cytometry. It has also been reported to work in Western Blot, immunoprecipitation and immunohistochemistry (frozen and paraffin).______________________________________________________________________________________________________Flow Cytometry AnalysisBalb/c whole blood (left) or bone marrow (right) were stained with anti-Ly 76 (clone: TER119) (filled histogram) or rat IgG2b isotype control (open histogram).
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