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Anti-Human (CD45 (T200)), Alexa Fluor®488 (clone YAML 501.4), (Rat IgG2a)
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Product Code:
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CL7605AF4 |
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Supplier Name:
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Cedarlane |
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Size:
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100 ug |
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Data Sheet:
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View Data Sheet |
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Categories:
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YAML 501.4 |
Additional Product Details
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Applications:
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F |
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Clone:
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YAML 501.4 |
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Format:
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Alexa Fluor488 |
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Isotype:
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Rat IgG2a |
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Specificity:
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CD45 (T200) |
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Host:
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Rat |
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Conjugate:
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Alexa Fluor488 |
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Species Reactivity:
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Human |
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DescriptionCedarlane’s anti-Human CD45 monoclonal antibody specifically binds the human CD45 Leukocyte Common Antigen (T200). CD45 is a family of single-chain transmembrane glycoproteins that consists of at least four isoforms (220, 205, 190, 180 kDa). Anti-Human CD45 monoclonal antibody recognizes an epitope common to all known isoforms. CD45 is expressed on hematopoietic cell lines, but not mature red cells, and is not detected on differentiated cells of other tissues. The protein phosphatase activity of the intracellular portion of CD45 is involved in transmembrane signaling for regulation of various cellular functions. Antibody to CD45 is used to differentiate lymphomas from non-hematopoietic undifferentiated neoplasms.______________________________________________________________________________________________________Flow Cytometry Analysis
Human peripheral blood lymphocytes were stained with anti-CD45 (T200) (clone: YAML 501.4) (filled histogram) or Rat IgG2a isotype control (open histogram).______________________________________________________________________________________________________Immunofluorescence Analysis
Purified Anti-Human CD45 (T200) (CL7605AP) staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification with DAPI counterstain. (Rat anti-human CD45 (T200) (CL7605AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
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References
1) Aqel NM, Ball RY, Waldmann H, Mitchinson MJ. Identification of macrophages and smooth muscle cells in human atherosclerosis using monoclonal antibodies. J Pathol. 146(3):197-204, 1985.
2) Johnson P, Greenbaum L, Bottomly K, Trowbridge IS. Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. J Exp Med. Mar 1; 169 (3):1179-84. 1989.
3) Streuli M, Hall LR, Saga Y, Schlossman SF, Saito H. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J Exp Med. Nov 1; 166 (5):1548-66. 1987.
4) Hale G, Clark M, Waldmann H. Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. May; 134(5):3056-61. 1985.
