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Anti-Mouse NK Cells (NK1.1), APC (Clone PK136) (mouse IgG2a)
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Product Code:
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CL8994APC |
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Supplier Name:
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Cedarlane |
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Size:
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0.1 mg |
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Data Sheet:
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View Data Sheet |
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Categories:
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PK136 |
Additional Product Details
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Applications:
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F |
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Clone:
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PK136 |
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Format:
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APC |
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Isotype:
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Mouse IgG2a |
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Specificity:
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CD161 (NK1.1) |
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Host:
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Mouse |
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Conjugate:
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Allophycocyanin |
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Species Reactivity:
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Mouse |
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DescriptionCedarlane’s anti-mouse NK cells monoclonal antibody is specific for mouse NK cells in selected strains of mice (i.e.C57BL, FVB/N, NZB but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, SJL and 129). Clone PK136 is specific for mouse NK1.1, also known as NKR-PIC and Ly 55. Mouse NK1.1 is expressed on NK cells and NKT cells on the following strains: C57BL, FVB/N and NZB. There are published reports that PK136 mAb binds to NKR-PIB on SJL/K NK cells4.This antibody is suitable for use in flow cytometry and with paraffin-embedded sections.______________________________________________________________________________________________________Immunohistochemical Analysis
Biotin Anti-Mouse NK Cells (NK1.1) (CL8994B) staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 2.5x (Left) and 40x (Right) magnification. (Biotin Anti-mouse NK Cells (NK1.1) (CL8994B) was used at a dilution of 1:100 and stained with DAB for 1 minute)
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References
1) Koo, G.C. and Peppard, J.R. 1981. Establishment of monoclonal anti-NK-1.1 antibody, Hybridoma 3:301-303.
2) Koo, G.C., Dumont, F., Hackett, J.Jr,]., Tutt, M. and Kumar, V. 1986. The NK-1/1(-) mouse: A model to study differentiation of murine NK cells. J. Immunol. 137:3742-3737.
3) Kung, S.K.P., and Miller, R.G. 1985. The NK1.1 antigen in NK-mediated F1 antiparent killing in vitro. J. Immunol. 154:1624.
4) Kung, S.K.P., Ruey-Chyi, S., Shannon, J. and R. Miller. 1999. The NKR-P1B Gene product is an inhibitory receptor on SJL/J NK cells. J. Immunol. 162 (10) 5876.
5) Wang, M., et al, 1998. Natural Killer Cell Depletion Fails to Influence Initial CD4 T Cell Commitment In Vivo in Exogenous Antigen-Stimulated Cytokine and Antibody Responses. J. Immunol 160: 1098-1105.
6) Zhao, Y., Sergio, J., Swenson, K., Arn, J., et al, 1997. Positive and Negative Selection of Functionala Mouse CD4 Cells by Porcine MHC in Pig Thymus Grafts. 159: 2100-2107.
7) Williams, N.S., and Engelhard, V. 1997. Perforin-Dependent Cytotoxic Activity and Lymphokine Secretion by CD4+ T cells are Regulated by CD8+ T cells. J. Immunology 159: 2091-2099.
