Adult Mouse Hypothalamus Cell Line mHypoA-BMAL1-WT/M (mixed)

This product line is based on proprietary platform technology which has enabled the creation of a series of immortalized hypothalamic neurons. These neurons were immortalized via retroviral transfer of SV40 T-Ag into hypothalamic primary cultures isolated from BMAL1+/+ (wildtype littermate controls; WT) and BMAL1−/− (knockout; KO) C57BL/6J mice (males and females, bred from BMAL1+/− mice obtained from Jackson Laboratories; stock no. 009100).

These cell lines have been found to express an ever-expanding array of neuropeptides, enzymatic markers, and biologically active receptors. As such, these cell lines enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including circadian rhythm dysregulation, hypertension, diabetes, obesity, and metabolic disorders, amongst others.

Further details regarding these cell lines can be found in the following paper:

• Loganathan Endo 2019