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Direct-zol™ RNA MiniPrep (200 Preps.)
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Product Code:
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R2052 |
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Supplier Name:
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Zymo Research Corporation |
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Size:
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EA |
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Data Sheet:
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View Data Sheet |
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Tags:
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RNA, RNA PURIFICATION |
Additional Product Details
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Additional Details:
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The Direct-zol™ RNA MiniPrep Kits provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA.The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).The entire procedure typically takes only 7 minutes.,(Isolate total RNA and small/miRNA from TRIzol® without phase separation) |
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UNSPSC:
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41105518 |
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- Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
- Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.
Product Summary
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).
Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA (see below).
The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
The entire procedure typically takes only 7 minutes.
Specifications
- Equipment: Microcentrifuge
- Sample Sources: Any sample stored and preserved in TRI Reagent®, TRIzol® or similar. (animal cells, tissue, bacteria, yeast, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
- RNA Purity: A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA can be accomplished using an in-column DNase I digestion
- Compatibility: TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
- RNA Storage: RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors is highly recommended for prolonged storage.
- RNA Size Limits: RNAs ≥17 nucleotides.
- Sample Inactivation: TRI Reagent® (provided with R2051, R2053, R2061, R2063, R2071, R2073 only) inhibits RNase activity and inactivates viruses and other infectious agents.
