Adult Mouse GnRH/GFP Cell Line

mHypoA-GnRH/GFP Cell Line

mHypoA-GnRH/GFP was generated using a proprietary platform technology enabling the creation of a pool of immortalized hypothalamic neurons. These neurons were immortalized from GnRH-GFP transgenic mice hypothalamic primary cultures by retroviral transfer of SV40 T-Ag. The immortalized hypothalamic cells were sorted on a cell sorting flow cytometer based on GFP fluorescence to greater than 95% purity. These cells represent the entire population of mHypoA-NPY/GFP neurons and have not been further subcloned.

These cells have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors. As such, they enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.

The datasheet for these products can be downloaded here.

Further details regarding this cell line can be found in the following paper:

• Molecular and Cellular Endocrinology (2013) by McFadden et al.

Download the mHypoA-GnRH/GFP Cell Line Screen Profile

Supplementary Figures

The immortalized mHypoA-GnRH/GFP line co-expresses GFP and GnRH, as determined by ICC. The levels of GnRH expression are comparable to the GT1-7 cell line. To-PRO-3 is a nuclear stain, whereas the differential interference contrast (DIC) image is also shown. (McFadden et al. (2013) Mol Cell Endocrinol. 377(1-2): 65-74.)