Adult Mouse ORX/GFP Cell LInes

mHypoA‑ORX/GFP‑4 Cell Line

The mHypoA‑ORX/GFP‑4 cell line comprises immortalized hypothalamic neurons enriched for orexin (hypocretin) neurons and marked by GFP. The donor tissue was the hypothalamus of an 8‑week‑old female transgenic mouse expressing eGFP under the human prepro‑orexin (HCRT) promoter; this transgenic line was created by Takashi Sakurai (University of Tsukuba) and obtained via Luis de Lecea (Stanford University). The reporter line and promoter strategy are described by Yamanaka et al., 2003 (Neuron 38:701–713). Primary cultures were treated with ciliary neurotrophic factor (CNTF), transformed by retroviral SV40 large T‑antigen (neomycin‑resistant cassette) and selected in geneticin; GFP+ cells were then FACS‑sorted to generate the GFP‑enriched, non‑clonal mHypoA‑ORX/GFP‑4 pool.

These cells have been found to express an ever-expanding array of neuropeptides, enzymatic markers and biologically active receptors. As such, these cells enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.

Further details regarding these cell lines can be found in the following paper:

• Pintwala et al 2023

The datasheet for this product can be downloaded here.