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PGP 9.5

  • Immunohistochemical analysis of Paraffin Embedded Sections



    CL7755AP (Anti-Human PGP 9.5) staining (10 µg/ml) of a human cerebral cortex formalin-fixed, paraffin-embedded tissue section; seen at 10x (left) and 20x (right) magnification. Staining on normal brain cortex is negative. High grade tumor (glioma) shows sharp nuclear and cytoplasmic staining in more than 70% of the lesion.




    CL7756AP (Anti-Human PGP 9.5) staining (10 µg/ml) of a human cerebral cortex formalin-fixed, paraffin-embedded tissue section; seen at 20x (left) and 40x (right) magnification. Staining on normal brain cortex is negative. High grade tumor (glioma) shows sharp nuclear and cytoplasmic staining in more than 70% of the lesion.





    CL7756AP (Anti-Human PGP 9.5) staining (2 µg/ml) of a human colon formalin-fixed, paraffin-embedded tissue section; seen at 20x (left) and 40x (right) magnification. Cytoplasmic/membrane staining of peripheral nerve/ganglion and stromal cells are observed.





    CL7756AP (Anti-PGP 9.5) staining (0.1 µg/ml) of a rat paw tissue section. The tissue was frozen in M1 embedding matrix and cut on a cyrostat to 12 µm. It was fixed in a 0.1 M solution of PBS containing 0.2% (w/v) paraformaldehyde and 0.75% (w/v) picric acid. Intraepithelial nerve staining of PGP 9.5 is observed (green); tissue was counterstained with DAPI (blue). Image provided by Michael Anderson from Oklahoma State University, Center for Health Sciences. PhD student.



    Western Blot Analysis



    CL7755AP (0.1µg/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence




    CL7756AP (0.01µg/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


    Description

    Protein Gene Product (PGP) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. In recent years, antibodies to this protein have become established as markers for neurons and cells of the diffuse neuroendocrine system (DNES). PGP 9.5 appears to belong to a family of ubiquitin-carboxy terminal hydrolase isoenzymes, but while it shows activity towards synthetic ubiquitin esters, the in vivo substrate(s) of PGP 9.5 remain unknown. Monoclonal and polyclonal antibodies against PGP9.5 will stain neuronal cell bodies and axons in the central and peripheral nervous systems, small nerve fibers in nerve tissue, neuroendocrine cells in the pituitary, thyroid pancreas etc., and also tumors of the DNES. Antibodies to this protein marker can be applied in neuropathology, in experimental studies of innervations of peripheral tissues and in studies of the development and pathology of the DNES. It is emphasized that antibodies to PGP are particularly suitable for detecting small nerve fibers in peripheral tissues.

       
Product Name: Anti-Human PGP 9.5 (UCHL1) (199), (affinity purified) (Rabbit IgG)
Code:
CL7755AP
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$405.00 CDN
Product Name: Anti-Human PGP 9.5 (UCHL1) (199), (affinity purified) (Rabbit IgG)
Code:
CL7755AP-S
Supplier:
Cedarlane
Size:
20 ug
Unit Price:
$138.00 CDN
Product Name: Anti-Human PGP 9.5 (UCHL1) (184), (affinity purified) (Rabbit IgG)
Code:
CL7756AP
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$405.00 CDN
Product Name: Anti-Human PGP 9.5 (UCHL1) (184), (affinity purified) (Rabbit IgG)
Code:
CL7756AP-50
Supplier:
Cedarlane
Size:
50 ug
Unit Price:
$230.00 CDN
Product Name: Anti-Human PGP 9.5 (UCHL1) (184), (affinity purified) (Rabbit IgG)
Code:
CL7756AP-S
Supplier:
Cedarlane
Size:
20 ug
Unit Price:
$138.00 CDN
Product Name: Anti-Human PGP 9.5 (UCHL1) (184), (affinity purified) (mouse IgG)
Code:
CL7856AP
Supplier:
Cedarlane
Size:
50 ug
Unit Price:
$471.00 CDN
Product Name: Anti-Human PGP 9.5 (UCHL1) (184), (affinity purified) (mouse IgG)
Code:
CL7856AP-S
Supplier:
Cedarlane
Size:
15 ug
Unit Price:
$156.00 CDN

Custom Antibody Services

CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.