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Cedarlane › Reagents › Cedarlane Manufactured › Reagents For Rat › MHC Antibodies › RT1.A

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RT1.A

RT1.A

OX-18

  • Description

    Cedarlane's anti-rat RT1.A monoclonal antibody recognizes a monomorphic determinant of rat Class I MHC antigen (RT1.A) and thus reacts with all rat strains tested including AO(RT1µ), DA (RT1a), LEW (RT11) and PVG (RT1c). However, quantitative measurements suggest that only a subfraction of the total Class I molecules are recognized (2). Clone OX-18 labels all peripheral lymphocytes but only a sub-fraction of thymocytes. It may be that the weakly labeled cells do in fact express some RT1.A antigenic determinants but there is clearly a major quantitative difference amongst thymocytes. This monoclonal used in tissue sections preferentially labels lymphoid cells in the medulla of the thymus including those cells with the marker phenotype of mature T lymphocytes (2).
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    Flow Cytometry Analysis
    Fischer rat splenocytes were stained with anti-RT1.A (clone: OX-18) (filled histogram) or mouse IgG1, k isotype control (open histogram).
       
Product Name: Anti-Rat RT1.A Ascites (Clone OX-18) (mouse IgG1)
Code:
CL007A
Supplier:
Cedarlane
Size:
0.5ml
Unit Price:
$616.00 CDN
Product Name: Anti-Rat RT1.A Purified (Clone OX-18) (mouse IgG1)
Code:
CL007AP
Supplier:
Cedarlane
Size:
250ug
Unit Price:
$268.00 CDN
Product Name: Anti-Rat RT1.A Purified (Clone OX-18) (mouse IgG1)
Code:
CL007AP-2
Supplier:
Cedarlane
Size:
500ug
Unit Price:
$485.00 CDN
Product Name: Anti-Rat RT1.A Biotin (Clone OX-18) (mouse IgG1)
Code:
CL007B
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$258.00 CDN
Product Name: Anti-Rat RT1.A Biotin (Clone OX-18) (mouse IgG1)
Code:
CL007B-5
Supplier:
Cedarlane
Size:
500ug
Unit Price:
$633.00 CDN
Product Name: Anti-Rat RT1.A FITC (Clone OX-18) (mouse IgG1)
Code:
CL007F
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$258.00 CDN
Product Name: Anti-Rat RT1.A FITC (Clone OX-18) (mouse IgG1)
Code:
CL007F-5
Supplier:
Cedarlane
Size:
500ug
Unit Price:
$799.00 CDN
Product Name: Anti-Rat RT1.A No Azide (Clone OX-18) (mouse IgG1)
Code:
CL007NA
Supplier:
Cedarlane
Size:
1mg
Unit Price:
$812.00 CDN

  • References

    1)  Kearny, J.F., Radbruch, A., Leisegang, B., and K. Rajewsky. (1979) A New Mouse Myelona Cell Line That has lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines.  J. Immunol. 123, 1548-1550.

    2)  Fukumoto, T., McMaster, W.R. and A.F Williams. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens.  Two Ia antigens and expression of Ia and Class I antigens in rat thymus.  Eur. J. Immunol. 12, 237-243.

    3)  Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX-2 antigens.  Immunology. 42, 593-600.

Custom Antibody Services

CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.



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