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YTS 169.4
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DescriptionCedarlane's purified anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology1. It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro.This antibody has been reported to be suitable for use in immunohistochemistry on PFA-fixed paraffin-embedded tissue sections (antigen retrieval is required).7 This clone has also been reported to work in immunohistochemistry with frozen sections6.______________________________________________________________________________________________________Flow Cytometry Analysis
Balb/c mouse splenic T-cells (left) or thymocytes (right) were stained with anti-CD8a (clone: YTS 169.4) (filled histogram) or rat IgG2b isotype control (open histogram).
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Product Name:
Anti-Mouse CD8a (Ly 2) Ig fraction (Clone YTS 169.4) (rat IgG2b)
Code:
CL169A
Supplier:
Cedarlane
Size:
0.5ml
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Product Name:
Anti-Mouse CD8a (Ly 2) Alexa Fluor488 (Clone YTS 169.4) (rat IgG2b)
Code:
CL169AF4
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Mouse CD8a (Ly 2) Alexa Fluor647 (Clone YTS 169.4) (rat IgG2b)
Code:
CL169AF6
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Mouse CD8a (Ly 2) Alexa Fluor700 (Clone YTS 169.4) (rat IgG2b)
Code:
CL169AF7
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Mouse CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b)
Code:
CL169AP
Supplier:
Cedarlane
Size:
250ug
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Product Name:
Anti-Mouse CD8a (Ly 2) APC (Clone YTS 169.4) (rat IgG2b)
Code:
CL169APC
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Mouse CD8a (Ly 2), APC-Cy7 (Clone YTS 169.4) (rat IgG2b)
Code:
CL169APC-CY7
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Mouse CD8a (Ly 2) Biotin (Clone YTS 169.4) (rat IgG2b)
Code:
CL169B
Supplier:
Cedarlane
Size:
100ug
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Product Name:
Anti-Mouse CD8a (Ly 2) Biotin (Clone YTS 169.4) (rat IgG2b)
Code:
CL169B-3
Supplier:
Cedarlane
Size:
300ug
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Product Name:
Anti-Mouse CD8a (Ly 2) FITC (Clone YTS 169.4) (rat IgG2b)
Code:
CL169F
Supplier:
Cedarlane
Size:
100ug
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Unit Price: |
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References
1) Cobbald S.P et al. (1984) Nature. Therapy with monoclonal antibodies by elimination of T cell subsets in vivo 312, 548-551.
2) Cobbald S.P. et al. 8th International Conference on Lymphatic Tissues and Germinal Centres. Plenum Press (Ed. Klaus G.) in press (1984) Immunosuppression with monoclonal antibodies - rules for effective serotherapy.
3) Aqel N.M. et al. (1984) J. of Immunol. Methods. 69: 207-214. Immunohistological Screening in the selection of monoclonal antibodies: the use of isotype specific antiglobulins.
4) Ledbetter J.A. and Hertzenberg L.A. (1979) Nature. 277: 131-133. Rat x Rat hybrid myelomas and a monoclonal anti-Fd portion of mouse Ig.
5) Mueller, R. et al. (1997) J. of Immunol. 159: 1599-1603. IL-4 Expression by Grafts from Transgenic Mice Fails to Prevent Allograft Rejection.
6) Stevenson, P.G. et al. (1997) J. of Immunol. 159: 1876-1884. Virus Dissemination Through the Brain Parenchyma Without Immunologic Control.
7) Zhang B, Kracker S, Yasuda T, Casola S, et al. (2012) Immune Surveillance and Therapy of Lymphomas Driven by Epstein-Barr Virus Protein LMP1 in a Mouse Model. Cell. 148(4):739-51.
8) Beilke JN, Benjamin J, Lanier LL. (2010) The requirement for NKG2D in NK cell–mediated rejection of parental bone marrow grafts is determined by MHC class I expressed by the graft recipient. Blood. 116(24):5208-16.
9) Visan I, Tan JB, Yuan JS, Harper JA, Koch U, Guidos CJ. (2006) Regulation of T lymphopoiesis by Notch1 and Lunatic fringe–mediated competition for intrathymic niches. Nat Immunol. 7(6):634-43.
Custom Antibody Services
CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.
