20% OFF POPULAR PRODUCTS FROM LIST LABS
Tweet, Share, View all associated products with this promotion!
Nov 13 2018
Dec 31 2018
View a complete listing of all associated products with this promotion. Still need more help? Please contact customer service to relieve any additional questions you may have.
Bacterial Toxins for Research
Our company has always focused on quality bacterial toxins, which you can depend on for your research. Because toxins have been the basis for many vaccines, List Labs products have supported vaccine development. List Labs was the first to commercialize many bacterial toxins for research, including C.difficile toxins and pertussis toxin. Today, we remain uniquely focused on manufacturing, offering and supporting a wide range of bacterial products.
#152C Toxin A from C. Difficile
A major nosocomial pathogen, Clostridium difficile causes antibiotic-associated colitis and intestinal Inflammatory disease, together called CDI.
How does C Difficile Toxin cause diarrhea? By releasing two large protein enterotoxins, C difficileToxin A (TcdA) and C difficile Toxin B (TcdB), Clostridium difficile mediates inflammatory diarrhea and compromises intestinal epithelial cells. Either toxin when administered to cultured cells disrupts the cytoskeleton and sets off capsase-dependent apoptosis, although, C difficile Toxin B is many times more potent than C difficile Toxin A. Both toxins also activate intestinal epithelial and immune cells to produce cytokines and chemokines, likely contributing to the complications associated with severe CDI. Intracellular targets of TcdA and TcdB are RhoGTPases which regulate many host cell processes including establishing an epithelial barrier and the migration and signaling of immune cells. When the Rho proteins are glycosylated by C difficile Toxins A or B, signaling is disrupted leading to the intestinal damage and inflamation characteristic of CDI.
List Labs C. Difficile Toxins have been involved with the research and development of diagnostics.
#179A Pertussis Toxin (in glycerol)
Pertussis toxin (PT), a virulence factor produced by Bordetella pertussis, is a multi-subunit toxin which binds to most cultured mammalian cells and targets specific G protein, inhibiting the ability of the G protein to function in signaling pathways. Depending on the function of the G protein, the effects of PT can vary. This ability to inhibit pathways utilizing the Gi family of protein-coupled receptors is the basis of the use of PT as a tool in cell biology. PT plays a role in infection by suppressing and modulating various host immune responses to Bordetella pertussis. PT has been used to stimulate experimental autoimmune diseases in rodent models, such as experimental auto immune encephalitis (EAE).
Native pertussis toxin is provided as a consistent product in three different formulations. Pertussis toxin is lyophilized either in buffer (Product # 180) or in pure water (Product # 181). Additionally, pertussis toxin is sold in a glycerol solution (Product # 179).
List Labs sells a genetically inactivated form of pertussis toxin, Pertussis Toxin Mutant. This protein may be used as antigens and as carrier proteins for other antigens. Although an amino acid in the enzyme active site is altered and activity is diminished, this mutant retains some of the activity of the native toxin. For more details about the genetically inactivated toxin follow the link to Pertussis Toxin Mutant.
Pertussis toxin is a multi-component protein composed of six non-covalently bound subunits ranging in molecular weight from approximately 9 to 28 kDa. These subunits are designated as S1, S2, S3, S4 and S5 and occur in native pertussis toxin in a ratio of 1:1:1:2:1, where the subunit S4 is present in two copies. The largest subunit S1, also called the A protomer, is responsible for the ADP-ribosyltransferase activity; the A protomer alone will transfer the ADP ribose from NAD+ to a subunits of G proteins of the class Gai, Gao or Gat. The crystal structure of PTX reveals a pyramid-like shape with the A protomer situated on top of the S5 subunit which rests on two dimers, S2-S4 and S3-S4. Together the five subunit platform is called the B oligomer and under certain conditions PTX dissociates into just two parts, the enzymatic A protomer and the five subunit, binding complex, the B oligomer. This B oligomer allows PTX to enter most cells, attaching to glycan residues present on receptor proteins including TLR4 and glycoprotein Ib. After entering the cell via receptor-mediated endocytosis, PTX is transported retrogradely via the endosomal pathway and Golgi complex to the endoplasmic reticulum. A protomer is released from the toxin and translocates through the membrane of the endoplasmic reticulum where the toxin inactivates the target membrane-bound G proteins.
Bordetella pertussis virulence factors derived from native organisms and offered by List Labs, include Pertussis Toxin Subunits, Pertussis Toxin Mutant, Filamentous Hemagglutinin (FHA), Fimbriae 2/3, Pertactin (69 kDa protein), Adenylate Cyclase Toxin (ACT), Adenylate Cyclase Antigen and B. pertussis Lipopolysaccharide (LPS). More information about each of these products may be obtained through the links on the Bordetella Virulence Factors.
#187 Pertactin from Bordetella Pertussis
Pertactin (PRN) is a 69 kDa surface-located protein produced by Bordetella pertussis. Due to its immunogenic and protective properties, it is included in acellular pertussis vaccines. The contribution of this protein to Bordetella pathogenesis is under investigation; however, it appears to play a role in overcoming neutrophil-mediated clearance of Bordetella during early stages of establishing an infection. Pertactin is one of the most polymorphic B. pertussis proteins, occurring in the population in more than 13 variations. Pertactin offered by List is isolated from native cultures of B. pertussis strain 165.
Bordetella pertussis virulence factors derived from native organisms and offered by List Labs, include Pertussis Toxin, Pertussis Toxin Subunits, Pertussis Toxin Mutant, Filamentous Hemagglutinin (FHA), Fimbriae 2/3, Pertactin (69 kDa protein), Adenylate Cyclase Toxin (ACT), Adenylate Cyclase Antigen and B. pertussis Lipopolysaccharide (LPS). More information about each of these products may be obtained through the links on the Bordetella Virulence Factors.
Click here to search applicable products and start saving today!
Have questions? Need more information? Contact us at email@example.com to have all your questions answered in a timely and professional manner.
Note: This offer is not valid in conjunction with contract discounts or any other special pricing. Void where prohibited.