BLIRT is one of the only companies producing Proteinase K in NGS Grade.

Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates.

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. It is highly suited to this application since the enzyme is active in the presence of chemicals that denature proteins, such as SDSand urea, chelating agents such as EDTA, sulfhydryl reagents, as well as trypsin or chymotrypsin inhibitors. Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases. Some examples for applications: Proteinase K is very useful in the isolation of highly native, undamaged DNAs or RNAs, since most microbial or mammalian DNases and RNases are rapidly inactivated by the enzyme, particularly in the presence of 0.5–1% SDS.

Proteinase K MB GRADE

Recombinant Proteinase K from Tritirachium album expressed in Pichia pastoris, ideal for non-specific digesting of proteins in variety of applications. It is free of exonucleases and endonucleases. Fully active up to 56°C and under denaturing conditions (e.g. in the presence of urea and/or SDS). Solubility in water ≥20 mg/ml; Activity ≥ 30 U/mg lyophilizate; Specific activity ≥ 40 U/mg protein; ≥ 800 U/ml liquid; DNA content ≤ 10 pg/mg.

Proteinase K NGS Grade

The purest recombinant Proteinase K from Tritirachium album expressed in Pichia pastoris. Specially designed for non-specific digestion of proteins in diagnostics and NGS-grade applications requiring minimised residual DNA content.It is free of exonucleases and endonucleases. Fully active up to 56°C and under denaturing conditions (e.g. in the presence of urea and/or SDS). Solubility in water ≥50 mg/ml; Activity ≥ 35 U/mg lyophilizate; Specific activity ≥ 45 U/mg protein; DNA content ≤ 0.1 pg/mg.