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Hypothalamic
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Mouse and Rat Hypothalamic Cell Lines
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Imaging of Embryonic and Adult Hypothalamic Cell Lines
Examples of hypothalamic cell lines, both embryonic and adult, are illustrated within this figure.
(A-D) The embryonic mHypoE-46, -29/2, -38 and -43/5 were imaged using phase contrast microscopy. These four lines were used in studies described in the energy homeostasis section and the mHypoE-38 line was also used in studies described in the reproduction section.
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(E-I) The adult mHypoA-2/22, -2/1, -2/3 and -2/5 were imaged using confocal differential interference contrast microscopy.
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(J) The embryonic mHypoE-38 neurons were imaged using fluorescent confocal microscopy after immunocytochemical analysis with anti-ghrelin sera (green); nuclei were counterstained with propidium iodide (red).
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(K) The adult mHypoA-2/12 neurons were imaged using fluorescent microscopy after immunocytochemical analysis with an antibody against NPY (green); nuclei were counterstained with DAPI (blue).
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(L and M) The mHypoE-36/1 neurons were imaged using DAB staining during immunocytochemical analysis with antibodies against neurofilament (NF) and neurotensin (NT).
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Reference: C.M. Mayer et al., Hypothalamic cell lines to investigate neuroendocrine control mechanisms, Front. Neuroendocrinol. (2009), doi:10.1016/j.yfrne.2009.03.005
For information regarding additional markers not listed or to find out how these cell lines can easily be screened for additional peptides, receptors, and markers of interest, please contact us for more details.
Custom Antibody Services
CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.
