Anti-Rat CD4 PE (Clone W3/25) (mouse IgG1)
DescriptionCedarlane’s anti-rat T helper cell monoclonal antibodies recognize a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages (1,2,3,10). The antigen recognized by this clone is a surface glycoprotein of Mr 48 000 - 52 000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. Clone W3/25 labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR) (2,4,6). Addition of W3/25 to the MLR, inhibited proliferation and blocks the production of IL-2 (4,5,6,9). T cells which mediate cytotoxicity and suppressor functions are not labelled. (Thus, cells labelled by this antibody are not labelled by MRC OX-8)Clone W3/25 is invaluable for separating T cell subsets for functional studies and for labelling cells in tissue sections. It has been used in studying the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease. (8)
This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 (CL002), which labels all T cells, as well as MRC OX-8 (CL004) and W3/25 (CL003) which label non-overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).______________________________________________________________________________________________________Flow Cytometry AnalysisWistar rat splenocytes (left) or lymph nodes (right) were stained with anti-CD4 (clone: W3/25) (filled histogram) or mouse IgG1 isotype control (open histogram).
1) Williams, A.F., Galfre, G. and C. Milstein. (1977) Cell, 12, 6330673. Analysis of cell surfaces by xenogenic myeloma hybrid antibodies: Differentiation antigens of rat lymphocytes.
2) Brideau, R.J., Carter, P.B., McMaster, W.R., Mason, D.W. and A.F. Williams. (1980) Eur. J. of Immunol., 10, 609-615. Two subsets of Rat T Lymphocytes defined with monoclonal antibodies.
3) Barclay, A.N. (1981) Immunology, 42, 593-600. The Localization of populations of lymphocytes defined with monoclonal antibodies in rat lymphoid tissues.
4) Cantrell, D.A., Robins, R.A. and R.W. Baldwin. (1982) Cell Immunol. 70, 367-372. Rat lymphocyte subsets: Cellular requirements for the generation of T cell growth factors.
5) Mason, D.W., Pugh, C.W. and M. Webb. (1981) Immunology, 44, 75-87. The Rat Mixed Lymphocyte Reaction: Roles of a dendritic cell in intestinal lymph and T lymphocyte subset.
6) Webb, M., Mason, D.W. and A.F. Williams. (1979) Nature, 282, 841-843. Inhibition of mixed lymphocyte response by monoclonal antibody specific for rat T lymphocyte subset.
7) Dallman, M.J., Mason, D.W. and M. Webb. (1982) Eur. J. Immunol. 12, 511-518. The role of host and donor cells in the rejection of skin allografts by T cell deprived rats injected with syngenic T cells.
8) Mason, D.W. (1981) Transplantation, 32, 222-226. Subsets of T cells in the rat mediating lethal graft vs. host disease.
9) White, R.A.H., Mason, D.W., Williams, A.F., Galfre, G. and C. Milstein. (1978) J. Exp. Med. 148, 644-673. T Lymphocyte heterogeneity in the rat: Separation of functional sub-populations using a monoclonal antibody.
10) Jefferies, W.A., Green, J.R. and A.F. Williams. (1985) J. Exp. Med. 162, 117-127. Authentic T Helper CD4 (W3/25) antigen on rat peritoneal macrophages.
11) Whiteland, J.L et al (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.12) Tamatani, T. et al. (1991) Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies. Eur. J. Immunol. 21:627-633.