Anti-Rat CD8a Biotin (Clone OX-8) (mouse IgG1)
DescriptionCedarlane's anti-rat T cytotoxic/suppressor cell monoclonal antibodies recognize a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine (1,2,3,4). The antigen recognized is a complex of surface glycoproteins of Mr 34, 39, and 76 kDa and is the rat homologue of the human CD8 and the mouse Ly 2.3 antigen (5,8). Clone OX-8 labels all peripheral T cells that are unlabelled by clone W3/25 (CL003). It labels a T cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor (2). OX-8 and W3/25 can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft versus host disease (7). This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in rat, these being clone W3/13 (CL002) which labels all T cells, as well as clones W3/25 and OX-8 which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).______________________________________________________________________________________________________Flow Cytometry AnalysisWistar rat splenocytes (left) or thymocytes (right) were stained with anti-CD8a (clone: OX-8) (filled histogram) or mouse IgG1, k isotype control (open histogram).
1) Brideau, R.J., Carter, P.B., McMaster, W.R., Mason, D.W. and A.F. Williams. (1980) Eur. J. of Immunol. 10, 609-615. Two subsets of rat T lymphocytes defined with monoclonal antibodies.
2) Dallman, M.J., Mason, D.W. and M.Webb. (1982) Eur. J. Immunol. 12, 511-518. The role of host and donor cells in the rejection of skin allografts by T cell deprived rats infected with syngenic T cells.
3) Cantrell, D.A., Robins, R.A.,Brooks, C.G. and R.W. Baldwin. (1982) Immunology. 45, 97-103. Phenotype of rat natural killer cells defined by monoclonal antibodies marking rat lymphocyte subsets.
4) Lyscom,N. and M.J. Brueton. (1982) Immunology. 45, 775-783. Intraepithelial lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies.
5) Johnson, P., Gagnon, J., Barclay, A.N. and A.F. Williams. (1985) EMBO J. 4, 2539-2545. Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes.
6) Barclay,A.N. (1981) Immunology, 42, 593-600. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
7) Mason, D.W. (1981) Transplantation, 32, 22-226. Subsets of T cells in rat mediating lethal graft vs. host disease.
8) Thomas, M.L. and J.R. Green. (1983) Eur.J. Immunol. 13, 855-858. Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes; comparisons with mouse and human antigens.
9) Whiteland, J.L et al (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.