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Anti-Mouse CD4 APC (Clone YTS 191.1) (rat IgG2b)

Cedarlane
Product Code:
CL012APC
Supplier Name:
Cedarlane
Size:
100 ug
Data Sheet:
View Data Sheet
Categories:
YTS 191.1

Additional Product Details

Applications:
F
Clone:
YTS 191.1.2
Format:
APC
Isotype:
Rat IgG2b
Specificity:
CD4 (L3/T4; Ly4)
Host:
Rat
Conjugate:
Allophycocyanin
Species Reactivity:
Mouse
$280.00 CDN


  • Description

    The CD4 (L3/T4) antigen is thought to be the murine equivalent of the human Leu3/OKT4 antigen. This T cell surface molecule appears to be expressed by the helper/inducer subset of murine T cells and by delayed hypersensitivity T cells but not by cytotoxic T cells or their precursors. CD4 (L3/T4) and CD8a (Ly 2) have been shown to be present on mutually exclusive T cells in the peripheral lymphoid organs but the thymus contains cells expressing both CD4 (L3/T4) and CD8a (Ly 2).

    Cedarlane's anti-mouse CD4 (L3/T4) mAb binds to approximately 85% of mouse thymocytes, 20% splenocytes, 50% lymph node cells, and a small number of bone marrow cells. It detects a protein of approximately 52 kDa on SDS-PAGE "Western Blots" (from Con A blast cell membranes) and is therefore similar to a well characterized human Leu3/T4 antigen (4). 

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    Immunofluorescence Analysis


    Purified Anti-Mouse CD4 (CL012AP) staining of paraformaldehyde-fixed paraffin-embedded spleen sections at 10x (left) and 100x (right) magnification. (Rat anti-mouse CD4 mAb (CL012AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:100 (Molecular Probes) and Normal Goat Serum (blocking))

  • References

    1)   Cobbald S.P., Jayasuriya A., Nash A., Prospero T.D., & Waldmann H., (1984) Nature. Therapy with monoclonal antibodies by elimination of T cell subsets in vivo. 312, 5994, 548-551.

    2)   Cobbald S.P., Martin G., Lovat P.C., & Waldmann H., 8th International Conference on Lymphatic Tissues and Germinal Centres. Plenum Press (Ed. Klaus G.) in press (1984) Immunosuppression with monoclonal antibodies - rules for effective serotherapy.

    3)     Agel N.M., Clark M., Cobbald S.P. & Waldmann H. (1984) J. Immunol. Methods.  Immunohistological Screening in the selection of monoclonal antibodies: the use of isotype specific antiglobulins.  69, 207-214.

    4)     Dialynas D.P., et al. (1983) J. of Immunol.  Characterization of the murine T cell surface antigen designated L3/T4, identified by monoclonal antibody GK1.5. Similarity of L3/T4 to human Leu3/T4 molecule.  U131U, 2445-2451.

    5)     Mueller, R. et al.  (1997)  J. of Immunol. 159: 1599-1603. IL-4 Expression by Grafts from Transgenic Mice Fails to Prevent Allograft Rejection.


Cleardot