Anti-Mouse CD4 APC (Clone YTS 191.1) (rat IgG2b)
The CD4 (L3/T4) antigen is thought to be the murine equivalent of the human Leu3/OKT4 antigen. This T cell surface molecule appears to be expressed by the helper/inducer subset of murine T cells and by delayed hypersensitivity T cells but not by cytotoxic T cells or their precursors. CD4 (L3/T4) and CD8a (Ly 2) have been shown to be present on mutually exclusive T cells in the peripheral lymphoid organs but the thymus contains cells expressing both CD4 (L3/T4) and CD8a (Ly 2).
Cedarlane's anti-mouse CD4 (L3/T4) mAb binds to approximately 85% of mouse thymocytes, 20% splenocytes, 50% lymph node cells, and a small number of bone marrow cells. It detects a protein of approximately 52 kDa on SDS-PAGE "Western Blots" (from Con A blast cell membranes) and is therefore similar to a well characterized human Leu3/T4 antigen (4).
Immunofluorescence AnalysisPurified Anti-Mouse CD4 (CL012AP) staining of paraformaldehyde-fixed paraffin-embedded spleen sections at 10x (left) and 100x (right) magnification. (Rat anti-mouse CD4 mAb (CL012AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:100 (Molecular Probes) and Normal Goat Serum (blocking))
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4) Dialynas D.P., et al. (1983) J. of Immunol. Characterization of the murine T cell surface antigen designated L3/T4, identified by monoclonal antibody GK1.5. Similarity of L3/T4 to human Leu3/T4 molecule. U131U, 2445-2451.
5) Mueller, R. et al. (1997) J. of Immunol. 159: 1599-1603. IL-4 Expression by Grafts from Transgenic Mice Fails to Prevent Allograft Rejection.