Anti-Mouse CD8a (Ly 2) FITC (Clone YTS 169.4) (rat IgG2b)
DescriptionCedarlane's purified anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology1. It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro.This antibody has been reported to be suitable for use in immunohistochemistry on PFA-fixed paraffin-embedded tissue sections (antigen retrieval is required).7 This clone has also been reported to work in immunohistochemistry with frozen sections6.______________________________________________________________________________________________________Flow Cytometry AnalysisBalb/c mouse splenic T-cells (left) or thymocytes (right) were stained with anti-CD8a (clone: YTS 169.4) (filled histogram) or rat IgG2b isotype control (open histogram).
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