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Anti-Mouse CD8a (Ly 2) FITC (Clone YTS 169.4) (rat IgG2b)

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YTS 169.4

Additional Product Details

YTS 169.4
Rat IgG2b
CD8a (Ly 2; Lyt 2)
Species Reactivity:
$153.00 CDN

  • Description

    Cedarlane's purified anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology1. It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro

    This antibody has been reported to be suitable for use in immunohistochemistry on PFA-fixed paraffin-embedded tissue sections (antigen retrieval is required).7 This clone has also been reported to work in immunohistochemistry with frozen sections6.

    Flow Cytometry Analysis
    Balb/c mouse splenic T-cells (left) or thymocytes (right) were stained with anti-CD8a (clone: YTS 169.4) (filled histogram) or rat IgG2b isotype control (open histogram). 

  • References

    1)  Cobbald S.P et al. (1984) Nature. Therapy with monoclonal antibodies by elimination of T cell subsets in vivo 312, 548-551.

    2)  Cobbald S.P. et al. 8th International Conference on Lymphatic Tissues and Germinal Centres. Plenum Press (Ed. Klaus G.) in press (1984) Immunosuppression with monoclonal antibodies - rules for effective serotherapy.

    3)  Aqel N.M. et al. (1984) J. of Immunol. Methods. 69: 207-214. Immunohistological Screening in the selection of monoclonal antibodies: the use of isotype specific antiglobulins.

    4)  Ledbetter J.A. and Hertzenberg L.A. (1979) Nature. 277: 131-133. Rat x Rat hybrid myelomas and a monoclonal anti-Fd portion of mouse Ig.

    5)  Mueller, R. et al. (1997) J. of Immunol. 159: 1599-1603. IL-4 Expression by Grafts from Transgenic Mice Fails to Prevent Allograft Rejection.

    6)  Stevenson, P.G. et al. (1997) J. of Immunol. 159: 1876-1884. Virus Dissemination Through the Brain Parenchyma Without Immunologic Control.

    7)  Zhang B, Kracker S, Yasuda T, Casola S, et al. (2012) Immune Surveillance and Therapy of Lymphomas Driven by Epstein-Barr Virus Protein LMP1 in a Mouse Model. Cell. 148(4):739-51.

    8)  Beilke JN, Benjamin J, Lanier LL. (2010) The requirement for NKG2D in NK cell–mediated rejection of parental bone marrow grafts is determined by MHC class I expressed by the graft recipient. Blood. 116(24):5208-16.

    9)  Visan I, Tan JB, Yuan JS, Harper JA, Koch U, Guidos CJ. (2006) Regulation of T lymphopoiesis by Notch1 and Lunatic fringe–mediated competition for intrathymic niches. Nat Immunol. 7(6):634-43.