Anti-human syntaxin, FITC, (clone SP6), (Mouse IgG1)
Syntaxin 1 is a 35 KDa integral membrane protein that possesses a single C-terminal transmembrane domain, a SNARE domain (H3) and an N-terminal regulatory domain (Habc). Two isoforms of Syntaxin 1 have been identified, Syntaxin 1A and Syntaxin1B. Syntaxin 1A localizes to nerve terminals of sensory neurons and nerve fibres reaching small blood vessels. Whereas Syntaxin 1B is localized to motor end plates and muscle spindles.
Syntaxin 1A resides at the pre-synaptic membrane and is a key component of the SNARE core complex that also includes Synaptobrevin-2 and SNAP25. Several other proteins including synaptotagmin-1, Munc18-1 and complexin also bind this complex to form the functional SNARE complex. SNARE is responsible for driving secretory vesicle docking, fusion and ultimately exocytosis.
Purified Anti-Human Syntaxin (CL2706AP) staining of paraformaldehyde-fixed paraffin-embedded human cerebellum sections at 200x (A) and 400x (B) magnification with DAPI counterstain. (Mouse anti-human Syntaxin (CL2706AP) 1:500; Alexa Fluor 488 Goat anti-mouse 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
Anti-human Syntaxin-1, Alexa Fluor488, (clone SP6), (CL2706AF4) staining on Embryonic Mouse Hippocampal Cell Line mHippoE-5 cells (CLU197) imaged under confocal microscope with the 40X oil objective; nuclei were counterstained with DAPI (blue).
1) De Wit, H et al. Synaptotagmin-1 docks secretory vesicles to Syntaxin-1/SNAP-25 acceptor complexes. Cell. 138, 935-946, 2009.
2) Honer, WG et al. Cingulate cortex synaptic terminal proteins and neural cell adhesion molecule in schizophrenia. Neuroscience. 78(1), 99-110, 1997.
3) Honer, WG et al. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609(1-2), 9-20, 1993.
4) Honer, WG et al. Monoclonal antibodies to study the brain in schizophrenia. Brain Res. 500(1-2), 379-83, 1989.
5) Lang, T. and Jahn R. Core proteins of the secretory machinery. Handb. Exp. Pharmacol. (184): 107-27, 2008.6) Ungermann,C. and Langosch, D. Functions of SNAREs in intracellular membrane fusion and lipid bilayer mixing. J. Cell Science. 118, 3819-3828, 2005.