Anti-Mouse CD3e, FITC (Clone 145-2C11) (hamster IgG)
DescriptionCedarlane's anti-mouse CD3e monoclonal antibody is specific for a 25 kDa protein component (e-T3) of the antigen specific T cell receptor on all mouse strains tested. The e-T3 protein has been shown to be non-covalently associated on the cell surface ab heterodimer of the CD3 associated complex. This monoclonal antibody reacts with all mature T cells and can both activate and inhibit T cell function1. This fact identifies e-T3 as a cell surface protein involved in the transduction of activation signals. All peripheral T cells express this determinant however B cells and bone marrow cells have proven to be negative. Although the expression of this particular epitope on peripheral T cells is uniformly high, staining of thymocytes reveals distinct subpopulations of cells differing in the level of expression of this marker.This antibody will prove useful in studying the role of various components of the TCR complex in T cell activation and development, and will allow for the development of an animal model in which to investigate the immunoregulatory effects of in vivo administration of anti-CD3 antibodies, an area of obvious clinical importance. Anti-CD3e is ideal for flow cytometry applications, particularly as a specific marker for tracking mouse T cells. In addition, this monoclonal antibody, clone 145-2C11 was specifically designed to trigger T cell activation. This clone has also been reported to work in immunoprecipitation1,2 and Western Blotting8.______________________________________________________________________________________________________Flow Cytometry AnalysisC3H/He mouse splenic T-cells were stained with anti-CD3e (clone: 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram).
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