Complement Component C4 monoclonal antibody reacts with mouse C4 (and also with
C4b and C4d). The C4 component is a glycoprotein containing three polypeptide
C4 along with C1, C2 and C3 are all involved in the initial stages of
complement activation via the classical pathway. Upon binding of the C1 molecule
to the antibody coated target, C4 is activated when C1s hydrolyzes a small
fragment, C4a, from the amino terminus, exposing a binding site on the larger
fragment, C4b. The C4b fragment attaches to the target surface and the C2a
complement component attaches to the exposed binding site. The resulting C4b2a
complex is called C3 convertase, which converts the C3 proenzyme into its
active form. The C4a molecule is a peptide mediator of inflammation
of this antibody include immunofluorescence, flow cytometry, ELISA, Western
blots and immunohistochemistry on acetone fixed frozen sections.
Flow Cytometry Analysis
C57BL/6 mouse thymocytes incubated with
anti-mouse CD90 (Thy 1.2) (clone: 5a-8) and fresh mouse serum were stained with anti-mouse C4 (Clone: RmC16D2) (filled histogram) or rat IgG2a isotype
control (open histogram).