Anti-Human CD21, Biotin (clone:21A/5) (Mouse IgG1)
DescriptionCedarlane’s anti-Human CD21 monoclonal antibody specifically binds human CD21, also known as Complement component (3d/Epstein Barr virus) receptor 2 (CR2). CD21 is a protein expressed on mature B cells and follicular dendritic cells, and is weakly expressed on immature thymocytes and T lymphocytes. CD21 binds to C3d, C3dg, and iC3b in the complement system. Other ligands include Epstein Barr virus and Interferon alpha. CD21 plays a role in B cell activation by binding to CD23 and forming a co-receptor complex with CD19, CD81, and Leu13.______________________________________________________________________________________________________Flow Cytometry AnalysisHuman peripheral blood lymphocytes were stained with anti-CD21 (clone: 21A/5) (filled histogram) or Mouse IgG1 isotype control (open histogram).______________________________________________________________________________________________________Immunofluorescence AnalysisPurified Anti-Human CD21 (CL7613AP) staining of paraformaldehyde-fixed paraffin-embedded human tonsil sections at 200x (A) and 400x (B) magnification with DAPI counterstain. (Mouse anti-human CD21 mAb (CL7613AP) 1:500; Alexa Fluor 488 Goat anti-mouse 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
1) CD Guide for CD21 (1989) In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 1080. ISBN-13: 978-01926186722) Kovacs JM, Hannan JP, Eisenmesser EZ, Holers VM. (2009) Mapping of the C3d ligand binding site on complement receptor 2 (CR2/CD21) using nuclear magnetic resonance and chemical shift analysis. J Biol Chem. 284 (14):9513-20.