Anti-Human CD163, Biotin (clone: GHI/61) (mouse IgG1,k)
DescriptionCedarlane’s Anti-Human CD163 monoclonal antibody binds CD163, also known as haemoglobin scavenger receptor and M130. This receptor is a membrane glycoprotein of 130 kDa and is found during the late stages of inflammation exclusively on the surface of human monocytes and macrophages. CD163 is a member of the scavenger receptor cysteine-rich family, and is a receptor for the haemoglobin-haptoglobin complex.______________________________________________________________________________________________________Immunofluorescence AnalysisPurified Anti-Human CD163 (CL7617AP) staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification (A) and human tonsil sections at 200x (B) 400x (C) magnification with DAPI counterstain. (Mouse anti-human CD163 mAb (CL7617AP) 1:500; Alexa Fluor 488 Goat anti-mouse 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
Flow Cytometry Analysis
Human peripheral blood monocytes were stained with anti-CD163 (clone: GHI/61) (filled histogram) or mouse IgG1 isotype control (open histogram).
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3 Møller HJ, Peterslund NA, Graversen JH, Moestrup SK. Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 99(1):378-80. 2002.4) Moniuszko M, Kowal K, Rusak M, et al. Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 13(6):704-7. 2006.