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Anti-Human CD163, Biotin (clone: GHI/61) (mouse IgG1,k)

Cedarlane
Product Code:
CL7617B
Supplier Name:
Cedarlane
Size:
100 ug
Data Sheet:

View Data Sheet

Categories:
GHI/61

Additional Product Details

Applications:
WB, IP, C, P, F
Clone:
GHI/61
Format:
Biotin
Isotype:
Mouse IgG1,k
Specificity:
CD163
Host:
Mouse
Conjugate:
Biotin
Species Reactivity:
Human
$254.00 CDN


  • Description

    Cedarlane’s Anti-Human CD163 monoclonal antibody binds CD163, also known as haemoglobin scavenger receptor and M130. This receptor is a membrane glycoprotein of 130 kDa and is found during the late stages of inflammation exclusively on the surface of human monocytes and macrophages. CD163 is a member of the scavenger receptor cysteine-rich family, and is a receptor for the haemoglobin-haptoglobin complex.
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    Immunofluorescence Analysis  

    Purified Anti-Human CD163 (CL7617AP) staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification (A) and human tonsil sections at 200x (B) 400x (C) magnification with DAPI counterstain. (Mouse anti-human CD163 mAb (CL7617AP) 1:500; Alexa Fluor 488 Goat anti-mouse 1:500 (Molecular Probes) and Normal Goat Serum (blocking)) 


    Flow Cytometry Analysis



    Human peripheral blood monocytes were stained with anti-CD163 (clone: GHI/61) (filled histogram) or mouse IgG1 isotype control (open histogram).


  • References

    1)  Pulford K, Micklem K, McCarthy S, et al. A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 75(4):588-95. 1992.

    2)  Law SK, Micklem KJ, Shaw JM, et al. A new macrophage differentiation antigen which is a member of the scavenge receptor superfamily. Eur J Immunol. 23(9):2320-5. 1993.

    3  Møller HJ, Peterslund NA, Graversen JH, Moestrup SK. Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 99(1):378-80. 2002.

    4)  Moniuszko M, Kowal K, Rusak M, et al. Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 13(6):704-7. 2006.

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