Anti-Human C3/C3b/iC3b, FITC, (clone: 7C12) (Mouse IgG1)
DescriptionCedarlane’s anti-Human C3/C3b/iC3b monoclonal antibody specifically binds human C3 as well as the breakdown products C3b and iC3b. C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphylatoxic as well as various immunoregulatory properties.Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.Cedarlane’s CL7637 (clone: 1H8) and CL7636 (clone: 7C12) recognize separate, non-overlapping epitopes on C3 fragments. Clone 7C12 is suitable for use in Flow Cytometry and ELISA.______________________________________________________________________________________________________Flow Cytometry AnalysisBalb/c mouse thymocytes incubated with rabbit anti-mouse T cells and then incubated with fresh human serum were stained with anti-C3/C3b/iC3b (clone: 7C12) (filled histogram) or mouse IgG1 isotype control (open histogram).
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