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Anti-Mouse CD44, APC (Clone KM81) (rat IgG2a)

Cedarlane
Product Code:
CL8944APC
Supplier Name:
Cedarlane
Size:
100 ug
Data Sheet:

View Data Sheet

Categories:
KM81

Additional Product Details

Applications:
F
Clone:
KM81
Format:
APC
Isotype:
Rat IgG2a
Specificity:
CD44
Host:
Rat
Conjugate:
Allophycocyanin
Species Reactivity:
Mouse
$351.00 CDN


  • Description

    Cedarlane’s anti-mouse CD44 (Pgp-1, Ly-24) monoclonal antibody recognizes a 95 kDa glycoprotein found on most hematopoietic cells. It is thought to be important in the regulation of migratory properties of lymphocytes during development and the regulation of the interaction with bone marrow stromal cells during hematopoiesis. CD44 functions as a receptor for hyaluronate, although some cells expressing CD44 do not bind hyaluronate.

    This antibody has been shown to inhibit the growth of lymphoid and myeloid cells in long term bone marrow cultures. It also blocks the adhesive interactions of B cell hybridomas to a cloned stromal line or to hyaluronate coated dishes.
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    Flow Cytometry Analysis
    ATH mouse bone marrow stained with anti-CD44 (clone: KM81) (filled histogram) or Rat IgG2a isotype control (open histogram).
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    Immunofluorescence Analysis

     

    Purified Anti-Mouse CD44 (CL8944AP) staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 400x magnification with DAPI counterstain. (Rat anti-mouse CD44 mAb (CL8944AP) 1:100; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))

  • References

    1)  Lynch, F., and Ceredig R. Ly-24 (Pgp-1) expression by thymocytes and peripheral T cells. Immunol. Today 9:7.0.

    2)  Picker, L.J., De Pos Toyos J, Telen MJ et al. Monoclonal antibodies against CD44 [In (Lu)-related P80], and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. J. Immunol. 1989: 142:2046-51.

    3)  Miyake K, Medina K, Hayashi S-I et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long term bone marrow cultures. J. Exp. Med. 1990; 171:477-488.

    4)  Miyake K, Underhill CB, Lesley J. et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med 1990; 172:69-75.

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