Anti-Mouse CD44, APC (Clone KM81) (rat IgG2a)
DescriptionCedarlane’s anti-mouse CD44 (Pgp-1, Ly-24) monoclonal antibody recognizes a 95 kDa glycoprotein found on most hematopoietic cells. It is thought to be important in the regulation of migratory properties of lymphocytes during development and the regulation of the interaction with bone marrow stromal cells during hematopoiesis. CD44 functions as a receptor for hyaluronate, although some cells expressing CD44 do not bind hyaluronate.This antibody has been shown to inhibit the growth of lymphoid and myeloid cells in long term bone marrow cultures. It also blocks the adhesive interactions of B cell hybridomas to a cloned stromal line or to hyaluronate coated dishes.______________________________________________________________________________________________________Flow Cytometry AnalysisATH mouse bone marrow stained with anti-CD44 (clone: KM81) (filled histogram) or Rat IgG2a isotype control (open histogram).______________________________________________________________________________________________________Immunofluorescence AnalysisPurified Anti-Mouse CD44 (CL8944AP) staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 400x magnification with DAPI counterstain. (Rat anti-mouse CD44 mAb (CL8944AP) 1:100; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
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