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Anti-Mouse Granulocytes (Gr-1), APC (Clone RB6-8C5) (rat IgG2b)

Cedarlane
Product Code:
CL8991APC
Supplier Name:
Cedarlane
Size:
0.1 mg
Data Sheet:

View Data Sheet

Categories:
RB6-8C5

Additional Product Details

Applications:
F
Clone:
RB6-8C5
Format:
APC
Isotype:
Rat IgG2b
Specificity:
Granulocytes (Gr-1, Ly 6G)
Host:
Rat
Conjugate:
Allophycocyanin
Species Reactivity:
Mouse
$295.00 CDN


  • Description

    Cedarlane's anti-mouse Gr-1 monoclonal antibody reacts with the myeloid differentiation antigen Gr-1 (1,2). This 25-30 kDa cell surface antigen is expressed on myeloid cells but not lymphoid or erythroid cells. The expression of the Gr-1 antigen increases with granulocyte maturation (3) as shown by the distinct populations of bone-marrow cells this monoclonal antibody labels: negative, low positive and high positive.  Expression is transient on cells of monocytic lineage (3).

    CL8991AP is a useful antibody for studies of myeloid differentiation stages and their regulations by cytokines. Applications include flow cytometry (1,2,3) complement-mediated depletion (4) Western blot staining (5) and both frozen and paraffin sections (6).
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    Flow Cytometry Analysis
    Balb/c mouse bone marrow was stained with anti-Granulocytes (Gr-1) (clone: RB6-8C5) (filled histogram) (gated on high SSC population) or rat IgG2b isotype control (open histogram).
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    Immunofluorescence Analysis


    Purified Anti-Mouse Granulocytes (Gr-1) (CL8991AP) staining of paraformaldehyde-fixed paraffin-embedded human tonsil sections at 200x (A) and 400x (B) magnification with DAPI counterstain. (Rat anti-mouse Granulocytes (Gr-1) (CL8991AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))

  • References

    1)  Spangrude, G.J., et al. Purification and characterization of mouse hematopoietic stem cells. Science 241:58-62, 1991.

    2)  Muller, C. E. et al. Isolation of two early B lymphocyte progenitors from mouse marrow: a committed pre-pre-B cell and a clonogenic thy-1 lo hematopoietic stem cell. Cell 44:653-662. 1986.

    3)  Kjetil, H. et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J. Immunol. 147: 22-28. 1991.

    4)  Brummer, Elmer et al. Immunological activation of polymorphonuclear neutrophils for fungal killing: Studies with murine cells and blastomyces dermatitidis in vitro. J. Leuko. Bio 36:505-520. 1984.

    5)  Jutila, M.A. et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. J. Immunol. 18: 1819-1826, 1988.

    6)  Whiteland, J.L et al (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.


    7)  Haestdal, K., et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cell. J.Immunol. 147:22. 1991.


    8)  Fleming, T.J., M.L. Fleming, and T.R. Malek. 1993. Selective Expression of Ly-6G on myeloid lineage cells in bone marrow. RB6-85 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 151:139.


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