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145-2C11

  • Description

    Cedarlane's anti-mouse CD3e monoclonal antibody is specific for a 25 kDa protein component (e-T3) of the antigen specific T cell receptor on all mouse strains tested. The e-T3 protein has been shown to be non-covalently associated on the cell surface ab heterodimer of the CD3 associated complex. This monoclonal antibody reacts with all mature T cells and can both activate and inhibit T cell function1.  This fact identifies e-T3 as a cell surface protein involved in the transduction of activation signals. All peripheral T cells express this determinant however B cells and bone marrow cells have proven to be negative. Although the expression of this particular epitope on peripheral T cells is uniformly high, staining of thymocytes reveals distinct subpopulations of cells differing in the level of expression of this marker.

    This antibody will prove useful in studying the role of various components of the TCR complex in T cell activation and development, and will allow for the development of an animal model in which to investigate the immunoregulatory effects of in vivo administration of anti-CD3 antibodies, an area of obvious clinical importance. Anti-CD3e is ideal for flow cytometry applications, particularly as a specific marker for tracking mouse T cells.  In addition, this monoclonal antibody, clone 145-2C11 was specifically designed to trigger T cell activation. This clone has also been reported to work in immunoprecipitation1,2 and Western Blotting8.
    ______________________________________________________________________________________________________

    Flow Cytometry Analysis
    C3H/He mouse splenic T-cells were stained with anti-CD3e (clone: 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram). 
Found 11 Products
Showing Page 1 of 2
  • 1
  • 2
       
Product Name: Anti-Mouse CD3e, Ascites (Clone 145-2C11) (hamster IgG)
Code:
CL7202A
Supplier:
Cedarlane
Size:
0.5ml
Unit Price:
$505.00 CDN
Product Name: Anti-Mouse CD3e, Alexa Fluor®488 (Clone 145-2C11) (hamster IgG)
Code:
CL7202AF4
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$302.00 CDN
Product Name: Anti-Mouse CD3e, Alexa Fluor®647 (Clone 145-2C11) (hamster IgG)
Code:
CL7202AF6
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$302.00 CDN
Product Name: Anti-Mouse CD3e, Alexa Fluor®700 (Clone 145-2C11) (hamster IgG)
Code:
CL7202AF7
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$318.00 CDN
Product Name: Anti-Mouse CD3e, Purified (Clone 145-2C11) (hamster IgG)
Code:
CL7202AP
Supplier:
Cedarlane
Size:
250ug
Unit Price:
$254.00 CDN
Product Name: Anti-Mouse CD3e, Purified (Clone 145-2C11) (hamster IgG)
Code:
CL7202AP-2
Supplier:
Cedarlane
Size:
500ug
Unit Price:
$374.00 CDN
Product Name: Anti-Mouse CD3e, Biotin (Clone 145-2C11) (hamster IgG)
Code:
CL7202B
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$149.00 CDN
Product Name: Anti-Mouse CD3e, Biotin (Clone 145-2C11) (hamster IgG)
Code:
CL7202B-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$374.00 CDN
Product Name: Anti-Mouse CD3e, FITC (Clone 145-2C11) (hamster IgG)
Code:
CL7202F
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$166.00 CDN
Product Name: Anti-Mouse CD3e, FITC (Clone 145-2C11) (hamster IgG)
Code:
CL7202F-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$374.00 CDN
Found 11 Products
Showing Page 1 of 2
  • 1
  • 2
  • References

    1)   Leo, O. et al.  1987. Proc. Natl Acad. Sci. USA 84: 1374-1378.

    2)   Portoles, P. et al. 1989. J. of Immunol. 142: 4168-4175.

    3)   Bluestone, J.A. et al. 1987. Nature. 326: 82-84.

    4)   Hirsch, R. et al. 1988. J. of Immunol. 140: 3766-3772.

    5)   Hirsch, R. et al. 1989 J. of Immunol. 142: 737-743.

    6)   Ernst, D.N. et al. 1989. J. of Immunol. 142: 1413-1421.

    7)   Flamand, V. et al. 1990. J. of Immunol. 144: 2875-2882.

    8)   Salvadori S. et al. 1994. J. of Immunol. 153: 5176-5182.

    9)   Denkers, E.Y. et al.  997. J. of Immunol. 159: 1903-1908.

    10) Brunmark, A. and A.M. O’Rourke. 1997.  J. of Immunol. 159: 1676-1685

    11) Lahvis G.P. and J. Cerny.  1997. J. of Immunol. 159: 1783-1793.

    12) Chao, C. et al.  1997. J. of Immunol. 159: 1686-16

    13) Chung, C.D. et al.  1997. J. of Immunol. 159: 1758-1766.

    14) Berg, N.N. and H. L. Ostergaard. 1997.  J. of Immunol. 159: 1753-1757.

Custom Antibody Services

CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.



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