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Adult Mouse Kiss/GFP Cell LInes

  • mHypoA-Kiss/GFP-1-4 Cell Lines

    The mHypoA-Kiss/GFP cell lines were generated using a proprietary platform technology enabling the creation of pools of immortalized hypothalamic neurons. Kiss/GFP transgenic mouse (Kiss-1tm1.1(cre/EGFP)Stei/J; strain (129S6/SvEvTac x C57BL/6NCr)F1) hypothalami were micro-dissected from 2-month old female or male mice isolating the ARC and AVPV nuclei individually for the female mice or using the entire hypothalamus for the male mice. These mice contain an eGFP transgene driven by the Kiss-1 promoter. These hypothalamic primary cultures were immortalized by retroviral transfer of the SV40 T-Ag. The four immortalized hypothalamic pools were sorted on a cell sorting flow cytometer based on GFP fluorescence. These cells represent the entire population of mHypoA-Kiss/GFP neurons from the ARC (mHypoA-Kiss/GFP-3) and AVPV (mHypoA-Kiss/GFP-4) for the female and all Kiss neurons for the male (mHypoA-Kiss/GFP-1-2) and have not been further sub-cloned.

    These cells have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors. As such, these cells enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.

    Further details regarding these cell lines can be found in the following paper:

    The datasheet for this product can be downloaded here.

       
Product Name: Adult Mouse Hypothalamus Cell Line mHypoA-Kiss/GFP-1 (male)
Code:
CLU505
Supplier:
Cedarlane
Size:
1 mL
Extra Notes:
Please inquire for pricing.MTA required.
Unit Price:
Contact us for pricing
 
Product Name: Adult Mouse Hypothalamus Cell Line mHypoA-Kiss/GFP-2 (male)
Code:
CLU506
Supplier:
Cedarlane
Size:
1 mL
Extra Notes:
Please inquire for pricing.MTA required.
Unit Price:
Contact us for pricing
 
Product Name: Adult Mouse Hypothalamus Cell Line mHypoA-Kiss/GFP-3 (female; ARC nuclei derived)
Code:
CLU507
Supplier:
Cedarlane
Size:
1 mL
Extra Notes:
Please inquire for pricing.MTA required.
Unit Price:
Contact us for pricing
 
Product Name: Adult Mouse Hypothalamus Cell Line mHypoA-Kiss/GFP-4 (female; AVPV nuclei derived)
Code:
CLU508
Supplier:
Cedarlane
Size:
1 mL
Extra Notes:
Please inquire for pricing.MTA required.
Unit Price:
Contact us for pricing
 

Custom Antibody Services

CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.



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