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RB6-8C5

  • Description

    Cedarlane's anti-mouse Gr-1 monoclonal antibody reacts with the myeloid differentiation antigen Gr-1 (1,2). This 25-30 kDa cell surface antigen is expressed on myeloid cells but not lymphoid or erythroid cells. The expression of the Gr-1 antigen increases with granulocyte maturation (3) as shown by the distinct populations of bone-marrow cells this monoclonal antibody labels: negative, low positive and high positive.  Expression is transient on cells of monocytic lineage (3).

    CL8991AP is a useful antibody for studies of myeloid differentiation stages and their regulations by cytokines. Applications include flow cytometry (1,2,3) complement-mediated depletion (4) Western blot staining (5) and both frozen and paraffin sections (6).
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    Flow Cytometry Analysis
    Balb/c mouse bone marrow was stained with anti-Granulocytes (Gr-1) (clone: RB6-8C5) (filled histogram) (gated on high SSC population) or rat IgG2b isotype control (open histogram).
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    Immunofluorescence Analysis


    Purified Anti-Mouse Granulocytes (Gr-1) (CL8991AP) staining of paraformaldehyde-fixed paraffin-embedded human tonsil sections at 200x (A) and 400x (B) magnification with DAPI counterstain. (Rat anti-mouse Granulocytes (Gr-1) (CL8991AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
       
Product Name: Anti-Mouse Granulocytes (Gr-1), Ascites (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991A
Supplier:
Cedarlane
Size:
0.5ml
Unit Price:
$505.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), Alexa Fluor®488 (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991AF4
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$302.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), Purified (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991AP
Supplier:
Cedarlane
Size:
250ug
Unit Price:
$236.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), APC (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991APC
Supplier:
Cedarlane
Size:
0.1 mg
Unit Price:
$322.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), Biotin (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991B
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$149.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), Biotin (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991B-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$374.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), FITC (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991F
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$152.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), FITC (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991F-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$374.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), No Azide (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991NA
Supplier:
Cedarlane
Size:
1 mg
Unit Price:
$499.00 CDN
Product Name: Anti-Mouse Granulocytes (Gr-1), PE (Clone RB6-8C5) (rat IgG2b)
Code:
CL8991PE
Supplier:
Cedarlane
Size:
50ug
Unit Price:
$158.00 CDN
  • References

    1)  Spangrude, G.J., et al. Purification and characterization of mouse hematopoietic stem cells. Science 241:58-62, 1991.

    2)  Muller, C. E. et al. Isolation of two early B lymphocyte progenitors from mouse marrow: a committed pre-pre-B cell and a clonogenic thy-1 lo hematopoietic stem cell. Cell 44:653-662. 1986.

    3)  Kjetil, H. et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J. Immunol. 147: 22-28. 1991.

    4)  Brummer, Elmer et al. Immunological activation of polymorphonuclear neutrophils for fungal killing: Studies with murine cells and blastomyces dermatitidis in vitro. J. Leuko. Bio 36:505-520. 1984.

    5)  Jutila, M.A. et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. J. Immunol. 18: 1819-1826, 1988.

    6)  Whiteland, J.L et al (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.


    7)  Haestdal, K., et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cell. J.Immunol. 147:22. 1991.


    8)  Fleming, T.J., M.L. Fleming, and T.R. Malek. 1993. Selective Expression of Ly-6G on myeloid lineage cells in bone marrow. RB6-85 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 151:139.

Custom Antibody Services

CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.



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