40% OFF KNOCKOUT LYSATES FROM NOVUS BIOLOGICALS
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Mar 28 2019
Jun 30 2019
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Limited Time Offer: Save on Novus Biologicals Knockout Lysates
In WB analysis, an initial indicator of antibody specificity is the presence of a single band at the expected target size, however proper antibody validation requires the use of adequate controls.
Because CRISPR/Cas9 gene-editing technology enables complete removal or “knock out” of both alleles of the gene encoding the target protein, antibody specificity is confirmed by demonstrating that a protein band is only present in the wildtype and not the KO cell lysate in WB analysis. To confirm differential expression, it is also important to analyze a separate target protein as a loading control (e.g., GAPDH) to demonstrate that all samples were loaded and transferred equally across wells.
Novus continues to support efforts geared at ensuring antibody specificity by adding more KO validated data to our antibody datasheets and adding a line of gene knockout reagents. Take your research a step further with our genome-wide selection of knockout cell lysates.
Useful applications include:
- In vitro Assays
- Functional Assays
- Simple Western
This promotion applies to select knockout lysates from Novus Biologicals. Please consult list of applicable products and terms & conditions prior to purchasing.
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Note: 40% off list price for Knockout Lysates from Novus Biologicals. Cannot be combined with other discounts or promotions, for prior purchases or for pending orders. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. Offer expires June 30, 2019.