20% OFF SIGNAL-SEEKER KITS FROM CYTOSKELETON
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May 15 2019
Jun 30 2019
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New Signal-Seeker™ SUMOylation 1 Detection Tools!
In mammalian cells, the small ubiquitin-like modifier (SUMO) family contains four isoforms (SUMO1, SUMO2, SUMO3, and SUMO4), and functions as a 12 kDa posttranslational modification (PTM) protein. SUMO2 and SUMO3 are nearly identical, differing in only three amino acid residues. SUMO1, also known as SMT3C, Sentrin, GMP1, UBL1, and PIC1, shares 48% identity with SUMO2/3 (1). SUMO4 is about 85% identical to SUMO2/3, but it is unclear whether SUMO4 can be conjugated to substrates (2). Proteins are post-translationally modified by SUMO conjugation (SUMOylation) to an acceptor lysine residue within a target protein consensus sequence ?KXE (where ? represents a hydrophobic amino acid and X represents any amino acid). Similar to ubiquitination, SUMOylation requires a three enzymes system (E1, E2, and E3) to conjugate SUMO covalently to target substrates.
The covalently linked SUMO can be removed by sentrin-specific proteases (SENPs), a process known as deSUMOylation (3). SUMOylation is a highly dynamic, reversible PTM that regulates the activity, subcellular localization, stability, and functions of target proteins and thereby modulates almost all major cellular pathways (4). As SUMO modifications are relatively new compared to other PTMs like ubiquitination or acetylation, tools to investigate SUMO are not well established. Appropriate lysis buffers, inhibitors, and high-quality affinity antibodies and beads are all critical reagents for effectively studying PTMs; in particular, SUMO modifications. Here we introduce Cytoskeleton’s highly validated SUMO 1 antibody and affinity beads, as well as the first comprehensive kit for endogenous SUMO1 detection (Cat. # BK165).
- Highest affinity and specificity antibody commercially available
- SUMO1 affinity beads is the only commercially affinity bead with exceptional specificity for SUMO1
- Comprehensive SUMO1 Detection kit is great for scientists that are new to the SUMO1 field
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