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50% OFF GSH DETECTION KITS

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Start Date:

Aug 1 2019

End Date:

Sep 23 2019


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Save 50% on Arbor Assays Glutathione Detection Kits through Cedarlane

August Promotion – Glutathione Detection Kits

Glutathione (GSH) Colorimetric Cuvette Detection Kit

The DetectX® Glutathione (GSH) Colorimetric Detection kit utilizes a colorimetric substrate that reacts with the free thiol group on GSH to yield a highly colored product. All reagents are in solution and require simple dilution for use in the assay. Free GSH concentration in the sample is calculated from the difference between the total GSH determined and the GSH generated from GSSG. The concentration of GSH can be determined either as an endpoint read of the color developed at 405nm after 30 minutes or by measuring the rate of color development at 405nm.

Glutathione (GSH) Fluorescent Detection Kit

The DetectX® Glutathione (GSH) Fluorescent Detection Kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar® and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Addition of a reaction mixture converts all the GSSG into free GSH, which then reacts with the excess ThioStar® during a second 15 minute incubation, yielding the signal related to Total GSH content.

Glutathione (GSH) Fluorescent Detection Kit (384-Well Plate)

The DetectX® Glutathione (GSH) Fluorescent Detection Kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar® and incubating at room temperature for 15 minutes. This kit uses 2 384-well plates that allow the measurement of about 375 samples in duplicate for either GSH or GSSG content, or measure about 183 samples in duplicate for both GSH and GSSG. The signal produced is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Sample GSSG is determined by addition of a reaction mixture converts all the GSSG into free GSH, which then reacts with ThioStar®.

Glutathione Reductase (GR) Fluorescent Activity Kit

The DetectX® Glutathione Reductase (GR) Fluorescent Activity Kit determines GR activity by the amount of GSH generated from the reduction of GSSG with a non-fluorescent molecule, ThioStar®, that covalently binds the free thiol group on GSH to yield a highly fluorescent product. The most widely used procedure to measure GR is to monitor the oxidation of NADPH as a decrease in absorbance at 340nm. However many biological molecules absorb light at 340 nm, plus the detection system gives very low OD readings. The Arbor Assays’ DetectX® Glutathione Reductase Fluorescent Activity kit overcomes these problems of measuring GR activity by the direct detection of the GSH formed from oxidized glutathione.

Glutathione S-Transferase (GST) Fluorescent Activity Kit

The DetectX® Glutathione S-Transferase (GST) Fluorescent Activity Kit is designed to quantitatively measure the activity of GST. A GST standard is provided to generate a standard curve for the assay. The kit utilizes a non-fluorescent molecule that is a substrate for the GST enzyme which covalently attaches to glutathione (GSH) to yield a highly fluorescent product. In the end point mode, mixing the sample or standard with the supplied Detection Reagent and GSH yields a fluorescent product which is read at 460nm in a fluorescent plate reader with excitation at 390nm. For rate determination, the intensity at 460nm is read periodically and plotted versus time.

Have questions? Need more information? Contact us at tech@cedarlanelabs.com to have all your questions answered in a timely and professional manner.

Note: Offer valid for direct purchases through made through Cedarlane. Some exclusions may apply. Offer cannot be used in conjunction with any other offers or discounts, for prior or past purchases, or orders pending. Limit one per customer. Offer void where prohibited. Some exceptions apply. Void where prohibited. Promotion Expires August 31st, 2019.

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