25% OFF CAYMAN'S ESTRADIOL ELISA KIT
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Aug 6 2019
Dec 31 2019
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Save on the NEW Estradiol ELISA Kit
A critical shortage in the antibody used in our Estradiol ELISA Kit (Item No. 582251) prompted the need for a replacement Estradiol ELISA Kit (Item No. 501890). Our scientists developed a new antiserum with less cross reactivity compared to the previous kit and changed the sample preparation/extraction protocol to a simpler and less hazardous format that provides improved specificity. Synthetic 17ß-estradiol and samples of human plasma were tested side by side in both kits to compare performance.
The new kit offers sensitivity values comparable to those of the previous kit. However, the concentrations of analyte or assay values of the new kit are detectable over a wider assay range compared to the previous kit.
Sample Preparation/Extraction Protocol
Plasma and serum samples may require extraction prior to quantification in the assay to eliminate potential substances like bulk proteins or lipids that may interfere with steroid quantification. Extraction can also help concentrate the sample to within the measurement range of the assay. We updated the extraction protocol in the new kit. This new protocol requires a single precipitation step with methanol, instead of the repeated liquid/liquid extraction steps needed with methylene chloride, which carries greater hazardous materials precautions.
- Previous Estradiol Kit (Item No. 582251) Protocol
- Methylene chloride used at 4X sample volume
- Extraction procedure is repeated 2 times
- New Estradiol Kit (Item No. 501890) Protocol
- Methanol used at 4X sample volume
- Precipitation procedure is one step
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Note: This offer is not valid in conjunction with contract discounts or any other special pricing. Void where prohibited.