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Start Date:

Nov 3 2020

End Date:

Jan 1 2021

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Save 20% on Active Motif TransAM Kits through Cedarlane

Back to the Lab Promotion – Save on Active Motif TransAM® Kits!

The TransAM® format is perfect for assaying transcription factor binding to a consensus-binding site. TransAM® Kits contain a 96-stripwell plate to which the consensus-binding site oligo has been immobilized. Activated nuclear extract is added to each well and the transcription factor of interest binds specifically to this bound oligonucleotide. A primary antibody specific for an epitope on the bound and active form of the transcription factor is then added followed by subsequent incubation with secondary antibody and Developing Solution to provide an easily quantified, sensitive colorimetric readout.

TransAM®?kits are sensitive, non-radioactive transcription factor ELISA-based assays that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. These optimized assays are available for many different transcription factors, including:

  • AP-1
  • ATF-2
  • c-Myc
  • C/EBP a/ß
  • CREB & pCREB
  • Elk-1
  • ER
  • FKHR (FOXO1)
  • GATA
  • GR
  • HIF-1
  • HNF
  • IRF-3
  • MAPK Family
  • MEF2
  • MyoD
  • NF-YA
  • NFATc1
  • NFkB
  • Nrf2
  • Oct-4
  • p53
  • PPARy
  • Sp1 and Sp1/Sp3
  • STAT3 and STAT Family
  • T-bet

Advantages of TransAM Transcription Factor ELISAs

Historically, transcription factor studies have been conducted using EMSAs/gel shift assays, western blots, and reporter-based assays with plasmid transfections, which are all time-consuming, do not allow for high-throughput analysis and provide only semi-quantitative results.

TransAM assays are up to 100 times more sensitive than gel shifts and can be completed in fewer than 5 hours. The TransAM assay is a non-radioactive ELISA-based assay, making them easier to perform. The high-throughput stripwell format enables simultaneous screening of up to 96 samples. Inconsistencies due to variable reporter plasmid transfection efficiencies are also eliminated, along with the need to construct stable cell lines.

Why Use TransAM Over Traditional Methods?

  • Sensitivity: Up to 100-fold more sensitive than gel shift assays
  • Convenience: Eliminates the use of radioactivity and the need to run gels
  • It's Fast: Results can be obtained in fewer than 5 hours
  • Ease of Analysis: Colorimetric readout enables simple quantitative analysis with spectrophotometry at 450 nm
  • Flexible Format: 96-stripwell format enables both high- and low-throughput assays
  • Active Motif TransAM Assay kits have been cited in more than 1,000 publications

Click here to download the ‘Reagents and Kits for Analysis of Protein-DNA Interactions’ brochure

Click here to view a complete listing of applicable products.

Note: Save 20% on orders of qualifying 1 x 96 rxns and 2 x 96 rxns TransAM kits. No other discounts apply. Other exclusions may apply. Offer subject to change at any time. This offer may not be applied to existing, pending, or prior orders. Promotion valid in CANADA only until December 31, 2020. This offer is not valid in conjunction with contract discounts or any other special pricing. Void where prohibited. Some restrictions apply. Contact your Cedarlane Sales or Customer Service Representative for further details.

Technology covered under EAT-filed patents and licensed to Active Motif. Use of TransAM in NF?B-related drug discovery may be covered under U.S. Patent No. 6,150,090 and require a license from Ariad Pharmaceuticals (Cambridge, MA, USA).