BUY A PRIMARY ANTIBODY, RECEIVE A FREE SECONDARY ANTIBODY!
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Oct 28 2020
Jan 1 2021
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Rockland and Cedarlane Special Offer – Stock Up and Save!
**To redeem this promotion, simply add a qualifying Rockland primary antibody and secondary antibody to your cart and the discount will automatically be applied.**
Primary antibodies are immunoglobulins that bind to a specific antigen (protein, peptide, small molecule, etc.) of interest and are typically unconjugated. A good primary antibody recognizes and binds with high affinity and specificity to purify, detect, and measure the antigen. Using a variety of hosts, such as mouse, rabbit, goat, or chicken, primary antibodies are available as polyclonal or monoclonal depending upon the application. Primary antibodies can be developed to recognize post-translational modifications (PTMs) including phosphorylation, methylation, glycosylation, or acetylation, making these antibodies great tools to study and identify proteins that are activated, silenced, processed, and/or may cause disease.
The assay format of these primary antibodies determines their application, i.e. unconjugated or conjugated to a reporter molecule (an enzyme or fluorophore). Conjugated primary antibodies can be useful because they eliminate the need for a detection secondary antibody.
Primary antibodies can also be used to recognize antigens in numerous types of assay formats including Western Blot, Enzyme-Linked Immunosorbent Assay (ELISA), Analytical Cytology, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Chromatin Precipitation (ChIP), Crystallography, Surface Plasmon Resonance (SPR), Neutralization, and others.
Primary Antibody Categories:
- Cell Biology
- Cell Cycle and Checkpoint Control
- Chromatin & Nuclear Signaling
- Developmental Biology
- DNA Damage & Repair
- Glucose & Energy Metabolism
- Post Translational Modifications
- Serum Protein Component
- Signal Transduction
- Stem Cell
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Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated.
Secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. The secondary antibody type is selected according to the class of the primary antibody (e.g., IgG or IgM), the source host, and the kind of label which is preferred. Most primary antibodies are of the IgG class and are produced in a common set of host species that includes rabbit, mouse, goat or chicken. Therefore, anti-mouse IgG, anti-rabbit IgG, anti-goat IgG or anti-chicken polyclonal antibodies are often used.
Choosing a secondary antibody is straightforward: select a secondary antibody that recognizes the host species used to produce the primary antibody of interest, i.e. choose an anti-mouse secondary antibody to detect a mouse monoclonal primary antibody. However, identifying the optimal secondary antibody requires knowledge of the detection assay. For example: Western blot and ELISA can be performed using colorimetric, chemiluminescent, and fluorescence reporter systems, while immunofluorescence and flow cytometry are generally limited to fluorescent reporter labels. In all of these instances, a conjugated secondary antibody is required.
Common Host-Specific Secondary Antibodies from Rockland:
- Anti-Rabbit Secondary Antibodies
- Anti-Goat Secondary Antibodies
- Anti-Chicken Secondary Antibodies
- Anti-Mouse Secondary Antibodies
- Anti-Donkey Secondary Antibodies
- Anti-Monkey Secondary Antibodies
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Have questions? Need more information? Contact us at firstname.lastname@example.org to have all your questions answered in a timely and professional manner.
Note: This offer is not valid in conjunction with contract discounts or any other special pricing. Void where prohibited. Purchase a full size Rockland primary antibody (including conjugated) to obtain a free qualifying secondary antibody. Trial size antibodies not applicable.