Custom Antibody Services
CEDARLANE® is an ISO 9001:2015 and ISO 13485:2016, MDSAP registered company that manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® has over 30 years of custom services experience having completed projects for a wide range of researchers, biotech manufacturers and pharmaceutical companies. We work with our customers to design and outline the specifics of the project, which are then initiated in a timely manner. Scheduling is communicated to the customer and we work to complete our projects on time, providing the excellent quality product that our customers have come to expect. At CEDARLANE®’s research facilities in Southern Ontario, we can accommodate projects of any scale; from 1 to 100+ animals, 1 mg to 1 gram of antibody, 1 to 1000+ test samples. CEDARLANE® understands the varied nature of custom work, and we encourage you to contact us to discuss the details of your project. All projects are kept strictly confidential.
Polyclonal Antibody Packages
CEDARLANE® offers complete project design including immunogen selection and production, antibody production, purification, conjugation, testing and lyophilization.
Host animals include:
rabbits, sheep, goats, mice, rats, guinea pigs, ferrets, llamas, chickens and more*.
Antigen sources include:
peptides, full or partial proteins, cells, lysates, plant material.
Our Standard Schedule is as follows:
Monoclonal Antibody Production
CEDARLANE® can supply monoclonal antibodies as Cell Line Supernatants (in vitro products), or as Ascites fluid using BALB/c, nude or other strains of mice.
Production includes initial cell expansion, priming of the host, isotyping and cryopreservation of the cells if required. Low endotoxin antibody production and purification is also available.
Monoclonal and polyclonal antibodies can be purified by:
- Protein G/A chromatography
- Affinity chromatography
- Ammonium Sulfate precipitation
All peptides are supplied with Mass Spectral analysis to confirm identity and HPLC chromatograms to ensure purity.
- Biotin, FITC, PE, Horseradish Peroxidase (HRP) and other dyes
- Carrier protein conjugations (for polyclonal antibody production; BSA, KLH, OVA)
Other conjugation services available upon request.
We can test your samples in ELISA, Flow Cytometry, Western Blot and Cytotoxicity assays.
Lyophilization/Freeze Drying Services
Material can be filled in specified sizes/vials and provided in a finished package format.
Bulk Antibody Production
CEDARLANE® also offers special pricing on bulk antibody orders. Most of our catalog antibodies are stocked in bulk amounts for quick delivery.
Custom Services References:
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Khorasani-Motlagh M, Lacasse MJ, Zamble DB. (2017) High-affinity metal binding by the Escherichia coli [NiFe]-hydrogenase accessory protein HypB is selectively modulated by SlyD.Metallomics. 9(5):482-493.
Tanner JR, et al. (2017) Legionella pneumophila OxyR Is a Redundant Transcriptional Regulator That Contributes to Expression Control of the Two-Component CpxRA System. J Bacteriol. 2017 Feb 14;199(5).
Gao J, Hay TJM, Banfield BW. (2017) The Product of the Herpes Simplex Virus 2 UL16 Gene Is Critical for the Egress of Capsids from the Nuclei of Infected Cells. J Virol. 91(10).
Esmail S, et al. (2016) N-Linked Glycosylation Is Required for Vacuolar H(+) -ATPase (V-ATPase) a4 Subunit Stability, Assembly, and Cell Surface Expression. J Cell Biochem. 117(12):2757-2768.
Tanner JR, Li L, Faucher SP, Brassinga AK. (2016) The CpxRA two-component system contributes to Legionella pneumophila virulence. Mol Microbiol. 100(6):1017-38.
Wong KK, Zhu F, Khatri I, Huo Q, Spaner DE, Gorczynski RM. (2016) Characterization of CD200 Ectodomain Shedding. PLoS One. 11(4):e0152073.
Kilmury SL, Burrows LL. (2016) Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS. Proc Natl Acad Sci U S A. 113(21):6017-22.
Henry KA, Tanha J, Hussack G. (2015) Identification of cross-reactive single-domain antibodies against serum albumin using next-generation DNA sequencing. Protein Eng Des Sel. 28(10):379-83.
Lamers RP, et al. (2015) Loss of membrane-bound lytic transglycosylases increases outer membrane permeability and β-lactam sensitivity in Pseudomonas aeruginosa. Microbiologyopen. 4(6):879-95.
Gene RW, et al. (2015) High affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires. J Immunol Methods. 416:29-39.
Nguyen, Ylan, et al. (2015) Pseudomonas aeruginosa minor pilins prime type IVa pilus assembly and promote surface display of the PilY1 adhesin.J Biol Chem. 290(1): 601-611.
Kandalaft, Hiba, et al. (2015) Targeting surface-layer proteins with single-domain antibodies: a potential therapeutic approach against Clostridium difficile-associated disease. Appl Microbiol Biotechnol. 1-14.
Shi C, Provost NB, Desroches T, Miller JD. (2014) Quantification of C. globosum spores in house dust samples. Ann Agric Environ Med. 21(3):525-30.
Desroches, T. C., D. R. McMullin, and J. D. Miller. (2014) Extrolites of Wallemia sebi, a very common fungus in the built environment. Indoor air 24(5): 533-542.
Douglas, Colin D., et al. (2013) Metal Transfer within the Escherichia coli HypB–HypA Complex of Hydrogenase Accessory Proteins. Biochemistry 52(35): 6030-6039.
Riley, Laura M., et al. (2013) Structural and Functional Characterization of Pseudomonas aeruginosa AlgX ROLE OF AlgX IN ALGINATE ACETYLATION. J Biol Chem. 288(31): 22299-22314.
Ochotny, Noelle, et al. (2013) The R740S mutation in the V‐ATPase a3 subunit results in osteoclast apoptosis and defective early‐stage autophagy. J Cell Biochem. 114(12): 2823-2833.
Voronov, Irina, et al. (2013) The R740S mutation in the V‐ATPase a3 subunit increases lysosomal pH, impairs NFATc1 translocation, and decreases in vitro osteoclastogenesis. J Bone Miner Res. 28(1): 108-118.
Salari S, et al. (2013) Extracellular HSP27 acts as a signaling molecule to activate NF-κB in macrophages. Cell Stress Chaperones 18(1):53-63.
Czesak M, et al. (2012) Increased serotonin-1A (5-HT1A) autoreceptor expression and reduced raphe serotonin levels in deformed epidermal autoregulatory factor-1 (Deaf-1) gene knock-out mice. J Biol Chem. 287(9):6615-27.
Krecisz S, Jones MD, Zamble DB. (2012) Nonspecific interactions between Escherichia coli NikR and DNA are critical for nickel-activated DNA binding. Biochemistry. 51 (40):7873-9.
Park J, Khuu N, Howard AS, et al. (2012) Bacterial- and plant-type phosphoenolpyruvate carboxylase isozymes from developing castor oil seeds interact in vivo and associate with the surface of mitochondria. Plant J. 71 (2):251-62.
Baldwin RM, Morettin A, Paris G, et al. (2012) Alternatively spliced protein arginine methyltransferase 1 isoform PRMT1v2 promotes the survival and invasiveness of breast cancer cells. Cell Cycle. 11 (24):4597-612.
Chan Chung KC, Zamble DB. (2011) Protein interactions and localization of the Escherichia coli accessory protein HypA during nickel insertion to [NiFe] hydrogenase. J Biol Chem. 286(50):43081-90.
Wehbi H, Portillo E, Harvey H, et al. (2011) The peptidoglycan-binding protein FimV promotes assembly of the Pseudomonas aeruginosa type IV pilus secretin. J Bacteriol. 193(2): 540-50.
Shi C, Belisle D, Miller JD. (2011) Quantification of the Aspergillus versicolor allergen in house dust. J lmmunol Methods. 372(1-2):89-94.
Gorczynski RM, et al. (2010) Breast cancer cell CD200 expression regulates immune response to EMT6 tumor cells in mice. Breast Cancer Res Treat. 123(2):405-15.
Finnen RL, Roy BB, Zhang H, Banfield BW. (2010) Analysis of filamentous process induction and nuclear localization properties of the HSV-2 serine/threonine kinase Us3. Virology. 397(1):23-33.
Dhanoa PK, Richardson LG, Smith MD, et al. (2010) Distinct pathways mediate the sorting of tail-anchored proteins to the plastid outer envelope. PLoS One. 5(4):e1 0098.
Wong KK, Khatri I, Shaha S, et al. (2010) The role of CD200 in immunity to B cell lymphoma. J Leukoc Biol. 88(2):361-72.
Harvey H, Habash M, Aidoo F, Burrows LL. (2009) Single-residue changes in the C-terminal disulfide-bonded loop of the Pseudomonas aeruginosa type IV pilin influence pilus assembly and twitching motility. J Bacteriol. 191(21):6513-24.
Tattoli I, Carneiro LA, Jehanno M, et al. (2008) NLRX1 is a mitochondrial NOD-like receptor that amplifies NF-kappaB and JNK pathways by inducing reactive oxygen species production. EMBO Rep. 9(3):293-300.
Goulet I, Boisvenue S, Mokas S, et al. (2008) TDRD3,a novel Tudor domain-containing protein, localizes to cytoplasmic stress granules. Hum Mol Genet. 17(19):3055-74.
Kus JV, Kelly J, Tessier L, et al. (2008) Modification of Pseudomonas aeruginosa Pa5196 type IV Pilins at multiple sites with D-Araf by a novel GT-C family Arabinosyltransferase,TfpW. J Bacteriol. 190(22):7464-78.
Sumarah MW, Miller JD, Adams GW. (2005) Measurement of a rugulosin-producingendophyte in white spruce seedlings. Mycologia. 97(4):770-6.
CEDARLANE® also offers:
- Special pricing on bulk (antibody) orders
- Custom formats and sizes
Please contact us for a quote: firstname.lastname@example.org or 1-800-268-5058 ext. 227 or 289-288-0001 ext. 227
CEDARLANE® continually endeavors to expand our line of antibodies. Please let us know if there is a specific antibody or clone that you are looking for. Please also inquire for special formats or sizes.
Still Need More Help?
If you have sales questions relative to particular products or to your line of research, please contact the CEDARLANE® sales department and have all of your questions answered in a timely and professional manner.