Anti-human syntaxin-1A, Purified, (clone SP8), (Mouse IgG1)
DescriptionSyntaxin 1 is a 35 KDa integral membrane protein that possesses a single C-terminal transmembrane domain, a SNARE domain (H3) and an N-terminal regulatory domain (Habc). Two isoforms of Syntaxin 1 have been identified, Syntaxin 1A and Syntaxin 1B. Syntaxin 1A localizes to nerve terminals of sensory neurons and nerve fibres reaching small blood vessels. Whereas Syntaxin 1B is localized to motor end plates and muscle spindles.
Syntaxin 1A resides at the pre-synaptic membrane and is a key component of the SNARE core complex that also includes Synaptobrevin-2 and SNAP25. Several other proteins including synaptotagmin-1, Munc18-1 and complexin also bind this complex to form the functional SNARE complex. SNARE is responsible for driving secretory vesicle docking, fusion and ultimately exocytosis.______________________________________________________________________________________________________Immunofluorescence AnalysisPurified Anti-Human Syntaxin (CL2707AP) staining of paraformaldehyde-fixed paraffin-embedded human cerebellum sections at 400x magnification with DAPI counterstain. (Mouse anti-human Syntaxin (CL2707AP) 1:500; Alexa Fluor 488 Goat anti-mouse 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
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