Low-Tox-H Rabbit Complement, Lyophilized (IVD)

Low-Tox^®-H Rabbit Complement for use with Human B-Lymphocytes

Low-Tox^®-H Rabbit Complement is a normal rabbit serum that has been processed for low toxicity to human peripheral blood B-lymphocytes. It is intended for use as a source of complement for HLA-DR tissue typing and all standard lymphocytotoxicity assays, particularly those that utilize cells sensitive to lysis by pooled normal rabbit serum. The cells are pre-incubated with the appropriate antiserum, then incubated with the complement source. After the appropriate time, the degree of cytotoxicity is scored. The conditions of testing will vary depending on the cell type and method of scoring. The standard microlymphocytotoxicity techniques are published in the NIAID Manual of Tissue Typing Techniques. It is important that appropriate complement controls be run in all assays as background toxicity will vary between systems.