Cedarlane › CL7501F

Anti-Mouse Complement Component C1q, FITC (Clone RmC7H8) (rat IgG1)

$323.00 CDN

Description

Cedarlane's FITC Anti-Mouse Complement Component C1q Monoclonal Antibody detects the C1q portion of the macromolecular C1 complex. C1 exists in the serum and consists of C1q and 2 molecules each of C1r and C1s, held together in a complex (C1qr2s2) stabilized by Ca2+ ions. C1q specifically binds surface bound IgG and IgM to facilitate complement activation via the classical pathway.
This clone has been reported to work in Flow Cytometry, immunofluorescence, staining of acetone fixed frozen sections, ELISA and Western Blotting (under reducing conditions, C1q falls apart into its three chains of 24, 25 and 28 kDa).
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Flow Cytometry Analysis
CBA/J mouse thymocytes incubated with rabbit anti-mouse T cells + fresh mouse serum + 5 mM EDTA were stained with anti-C1q (clone: RmC7H8) (filled histogram) or rat IgG1 isotype control (open histogram).

References
1) Schuh R, Kremmer E, Ego E, Wasiliu M and Thierfelder S. 1992. Determination of monoclonal antibody specificity by immunoadsorption and western blotting. J Immunol Methods 152(1): 59-67

2) Robson MG, Cook HT, Botto M, Taylor PR, Busso N, Salvi R, Pusey CD, Walport MJ and Davies KA. 2001. Accelerated nephrotoxic nephritis is exacerbated in C1q-deficient mice. J Immunol 166:6820

3) Trouw LA, Seelen MA, Duijs JMGJ, Benediktsson H, Van Kooten C and Daha MR. 2003. Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of anti-mouse C1q antibodies. Clin Exp Immunol 132: 32
4) Wasiliu M, Kremmer E et al. Monoclonal Antibodies to Complement Components Without the Need of Their Prior Purification. I. Antibodies to Mouse C1q. Hybridoma 8(6): 615-622

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