Anti-Rat CD45 Ascites (Clone OX-30) (mouse IgG2a)
DescriptionCedarlane's anti-rat CD45 monoclonal antibody recognizes a monomorphic determinant of the rat leukocyte common antigen (1). The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170,000 Da on thymocytes but molecular weight 170,000-220,000 Da on other leukocytes. The leukocyte common antigen (L-CA) is a major glycoprotein of haematopoietic cells but is not found on other tissues or erythroid cells. It is present on greater than 95% of thymocytes, bone marrow cells and thoracic duct lymphocytes. This molecule carries much of the carbohydrate of thymocytes and shows interesting heterogeneity amongst T lymphocytes and B lymphocytes (2,3).This antibody is suitable for use in flow cytometry and immunohistochemistry with frozen sections. This clone has also been reported to be unsuitable for use with paraffin sections.______________________________________________________________________________________________________Flow Cytometry AnalysisWistar rat thymocytes were stained with anti-CD45 (clone: OX-30) (filled histogram) or mouse IgG2a isotype control (open histogram).
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