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Anti-Mouse Complement Component C4 No Azide (Clone RmC16D2) (rat IgG2a)
$1289.00 CDN
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DescriptionCedarlane’s Anti-Mouse Complement Component C4 monoclonal antibody reacts with mouse C4 (and also with C4b and C4d). The C4 component is a glycoprotein containing three polypeptide chains (a, b and g). C4 along with C1, C2 and C3 are all involved in the initial stages of complement activation via the classical pathway. Upon binding of the C1 molecule to the antibody coated target, C4 is activated when C1s hydrolyzes a small fragment, C4a, from the amino terminus, exposing a binding site on the larger fragment, C4b. The C4b fragment attaches to the target surface and the C2a complement component attaches to the exposed binding site. The resulting C4b2a complex is called C3 convertase, which converts the C3 proenzyme into its active form. The C4a molecule is a peptide mediator of inflammation (anaphylatoxin).Reported applications of this antibody include immunofluorescence, flow cytometry, ELISA, Western blots and immunohistochemistry on acetone fixed frozen sections.______________________________________________________________________________________________________Flow Cytometry Analysis
C57BL/6 mouse thymocytes incubated with anti-mouse CD90 (Thy 1.2) (clone: 5a-8) and fresh mouse serum were stained with anti-mouse C4 (Clone: RmC16D2) (filled histogram) or rat IgG2a isotype control (open histogram).
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References1) Kremmer, E, Thierfelder S, Felber E, Hoffmann-Fezer, G, and Wasiliu, M. 1990. Monoclonal antibodies to complement componenets without the need of their prior purification. II. Antibodies to mouse C3 and C4. Hybridoma. Aug; 9 (4):309-17.
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