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Anti-Mouse C5a Receptor (CD88), PE (Clone 20/70) (rat IgG2b)
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Product Code:
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CL7588PE |
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Supplier Name:
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Cedarlane |
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Size:
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50 ug |
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Data Sheet:
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View Data Sheet |
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Categories:
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20/70 |
Additional Product Details
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Applications:
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F, IF, Blocking |
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Clone:
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20/70 |
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Format:
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PE |
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Isotype:
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Rat lgG2b |
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Specificity:
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CD88 (C5a receptor) |
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Host:
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Rat |
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Conjugate:
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R-phycoerythrin |
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Species Reactivity:
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Mouse |
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DescriptionThis monoclonal antibody detects the mouse C5a receptor (also known as CD88) found on peritoneal moncotyes/macarophages, alveolar macrophages, neutrophils and peripheral blood myeloid cells. This clone (20/70) abrogated neutrophil recruitment in the mouse peritoneum in vivo, blocked binding of FITC rC5a to murine C5aR and inhibited migration of transfectants (C5aR-RBL-2H3) in response to rC5a. Metal-induced apoptosis in transfected NIH3T3 cells was reduced with this antibody. Ligands for C5aR include the RP S19 dimer and C5a/C5a des Arg. Mouse C5a receptor has seven transmembrane segments and shares ~65% homology with human.Applications for this clone include flow cytometry, immunofluorescence and receptor blockage in vitro and in vivo. This antibody is not suitable for use in immunohistochemistry.______________________________________________________________________________________________________Flow Cytometry Analysis
Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate) (right) were stained with anti-C5a receptor (CD88) (clone: 20/70) (filled histogram) or rat IgG2b isotype control (open histogram).
CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.
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References
1) Soruri, A., et al. Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody. Immunology Letters, 88:47-52, 2003.
2) Shushakova, N., et al. C5a anaphylatoxin is a major regulator of activating versus inhibitory FcγRs in immune complex-induced lung disease. J. Clin. Invest. 110:1823-1830, 2002.
3) Godau, J., et al. C5a initiates the inflammatory cascade in immune complex peritonitis. J. of Immunol. 173: 3437-3445, 2004.
4) Nishiura, H., et al. S19 ribosomal protein dimer augments metal-induced apoptosis in a mouse fibroblastic cell line by ligation of the C5a receptor. J. of Cell. Biochem. 94:540-553, 2005.
5) Lee, H., et al. Human C5aR knock-in mice facilitate the production and assessment of anti-inflammatory monoclonal antibodies. Nature Biotechnology. 24(10):1279-84, 2006.
