Cedarlane › CL7611PE-SP

Anti-Human Complement Component C1q, PE (Clone MhC5B9) (mouse IgG1) (Special 100ug/0.5ml concentration)

$625.00 CDN

Description

Cedarlane's Anti-Human Complement Component C1q Monoclonal Antibody detects the C1q portion of the macromolecular C1 complex. C1 exists in the serum and consists of C1q and 2 molecules each of C1r and C1s, held together in a complex (C1qr2s2) stabilized by Ca2⁺ ions. C1q specifically binds surface bound IgG and IgM to facilitate complement activation via the classical pathway. The C1q subunit is made up of an umbrella-like radial array of six chains, each of which has a globular head connected by a collagen-like arm to a central stalk. The C1q molecule is 460 kDa (hexamer of three pairs of chains; 22, 23, 24 kDa). Note that the specific epitope (subunit) which this antibody recognizes has not yet been determined.
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Flow Cytometry Analysis
Balb/c mouse thymocytes incubated with anti-mouse CD90 (Thy 1.2) (clone: 5a-8) + fresh human serum + 5 mM EDTA were stained with anti-C1q (clone: MhC5B9) (filled histogram) or mouse IgG1 isotype control (open histogram).

References
1) Schuh R, Kremmer E, Ego E, Wasiliu M and Thierfelder S. (1992) Determination of monoclonal antibody specificity by immunoadsorption and western blotting. J Immunol Methods. 152(1): 59-67.

2) Zwirner J, Wittig A, Kremmer E, Gotze O. (1998) A novel ELISA for the evaluation of the classical pathway of complement. J. Immunol. Methods. 211: 183-190.
3) Nielsen C, Ostergaard O, Stener L, et al. (2012) Increased IgG on Cell-Derived Plasma Microparticles in Systemic Lupus Erythematosus Is Associated With Autoantibodies and Complement Activation. Arthritis and Rheumatism. 64(4):1227-36.

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