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Anti-Human CD59, (APC) (Clone 1F5) (mouse IgG1)

$628.00 CDN

Description

CD59 (also known as HRF20, protectin) is a 20 kDa glycoprotein attached to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It blocks MAC formation in the complement pathway by interfering with C9 membrane insertion and polymerization, but it does not affect the generation of C3 and C5 activation products. CD59 is expressed on several cell types, including leukocytes, endothelial and epithelial cells, erythrocytes, and cells of the central nervous system. CD59 belongs to the Ly6 superfamily of proteins.

This product is suitable for use in flow cytometry.
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Flow Cytometry Analysis
Human peripheral blood lymphocytes (left) or Red Blood Cells (right) were stained with anti-CD59 (clone: 1F5) (filled histogram) or Mouse IgG1 isotype control (open histogram).

References
1) Yu, J., et al. 1997. Mapping the active site of CD59. J. Exp. Med. 185 (4): 745-753.

2) Farkas, I., et al. 2002. CD59 blocks not only the insertion of C9 into MAC but inhibits ion channel formation by homologous C5b-8 as well as C5b-9. J. of Physiol. 539 (2): 537-545.

3) Zheng. H., et al. 1999. Targeting of functional antibody-CD59 fusion proteins to a cell surface. J. Clin. Invest. 103: 55-61.

4) Okada, N., et al. 1989. Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement. J. of Immuol. 143 (7): 2262-2266.

5) Yu, J., et al. 1997. The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase. Proc. Natl. Acad. Sci. 94: 12580-12585.

6) Chen, R., et al. 2000. Impaired growth and elevated Fas receptor expression in PIGA+ stem cells in primary paroxysmal nocturnal hemoglobinuria. J. Clin. Invest. 106: 689-696.

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