Canada
United States
International
Anti-Mouse Granulocytes (Gr-1), PE (Clone RB6-8C5) (rat IgG2b)
|
Product Code:
|
CL8991PE |
|
Supplier Name:
|
Cedarlane |
|
Size:
|
50ug |
|
Data Sheet:
|
View Data Sheet |
|
Categories:
|
RB6-8C5 |
Additional Product Details
|
Applications:
|
F |
|
Clone:
|
RB6-8C5 |
|
Format:
|
PE |
|
Isotype:
|
Rat IgG2b |
|
Specificity:
|
Granulocytes (Gr-1, Ly 6G) |
|
Host:
|
Rat |
|
Conjugate:
|
R-phycoerythrin |
|
Species Reactivity:
|
Mouse |
-
DescriptionCedarlane's anti-mouse Gr-1 monoclonal antibody reacts with the myeloid differentiation antigen Gr-1 (1,2). This 25-30 kDa cell surface antigen is expressed on myeloid cells but not lymphoid or erythroid cells. The expression of the Gr-1 antigen increases with granulocyte maturation (3) as shown by the distinct populations of bone-marrow cells this monoclonal antibody labels: negative, low positive and high positive. Expression is transient on cells of monocytic lineage (3).
CL8991AP is a useful antibody for studies of myeloid differentiation stages and their regulations by cytokines. Applications include flow cytometry (1,2,3) complement-mediated depletion (4) Western blot staining (5) and both frozen and paraffin sections (6).______________________________________________________________________________________________________Flow Cytometry Analysis
Balb/c mouse bone marrow was stained with anti-Granulocytes (Gr-1) (clone: RB6-8C5) (filled histogram) (gated on high SSC population) or rat IgG2b isotype control (open histogram).______________________________________________________________________________________________________Immunofluorescence Analysis
Purified Anti-Mouse Granulocytes (Gr-1) (CL8991AP) staining of paraformaldehyde-fixed paraffin-embedded human tonsil sections at 200x (A) and 400x (B) magnification with DAPI counterstain. (Rat anti-mouse Granulocytes (Gr-1) (CL8991AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
-
References
1) Spangrude, G.J., et al. Purification and characterization of mouse hematopoietic stem cells. Science 241:58-62, 1991.
2) Muller, C. E. et al. Isolation of two early B lymphocyte progenitors from mouse marrow: a committed pre-pre-B cell and a clonogenic thy-1 lo hematopoietic stem cell. Cell 44:653-662. 1986.
3) Kjetil, H. et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J. Immunol. 147: 22-28. 1991.
4) Brummer, Elmer et al. Immunological activation of polymorphonuclear neutrophils for fungal killing: Studies with murine cells and blastomyces dermatitidis in vitro. J. Leuko. Bio 36:505-520. 1984.
5) Jutila, M.A. et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. J. Immunol. 18: 1819-1826, 1988.
6) Whiteland, J.L et al (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.
7) Haestdal, K., et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cell. J.Immunol. 147:22. 1991.
8) Fleming, T.J., M.L. Fleming, and T.R. Malek. 1993. Selective Expression of Ly-6G on myeloid lineage cells in bone marrow. RB6-85 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 151:139.
