Cedarlane › CL7503APC

Anti-Mouse Complement Component C3, APC (Clone RmC11H9) (rat IgG2a)

$550.00 CDN

Description

Cedarlane’s Anti-Mouse Complement Component C3 monoclonal antibody reacts with mouse C3 as well as the breakdown products C3b, iC3b and C3dg. C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphylatoxic as well as various immunoregulatory properties.

Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.

Reported applications of this antibody include immunofluorescence^{2, 5}, ELISA⁴, Western blots¹, flow cytometry and immunohistochemistry^{1, 3} on acetone fixed frozen sections.
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Flow Cytometry Analysis
C3H/He mouse thymocytes incubated with anti-mouse CD90 (Thy 1.2) (clone: 5a-8) and fresh mouse serum were stained with anti-mouse C3 (clone: RmC11H9) (filled histogram) or rat IgG2a isotype control (open histogram).

References
1) Kremmer, E et al. (1990). Hybridoma. 9 (4):309-17.

2) Robson, M et al. (2001). J. Immunol. 166(11): 6820-6828.

3) Mastellos et al. (2004). Molecular Immunology 40: 1213-1221.

4) Zhili Xu et al. (2008). J. Virology. 82(23): 11705-13.
5) Binstadt, B et al. (2009). PNAS. 106(39): 16758-63.

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