Cedarlane › CL042AF6

Anti-Rat CD11b/c Alexa Fluor®647 (Clone OX-42) (mouse IgG2a)

$351.00 CDN

Description

Cedarlane’s anti-rat CD11b/c monoclonal antibody recognizes most macrophages including resident peritoneal macrophages, kupffer cells, but only approximately 35% of alveolar macrophages (2). It also labels dendritic cells extensively, granulocytes and cells with morphology of microglia in the brain. It precipitates three polypeptides of M.W. 160, 103 and 95kDa. OX-42 inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for iC3b called CR3. Cedarlane’s anti-rat CD11b/c mAb should prove useful in the recognition of macrophages and microglia.
This clone is reported to work with frozen and paraffin sections (3); in vivo and in vitro functional tests (4, 5, 6); and cryostat sections (5).
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Flow Cytometry Analysis
Wistar rat peritoneal macrophages were stained with anti-CD11b/c (clone: OX-42) (filled histogram) or mouse IgG2a isotype control (open histogram).

References
1) Aihara, N. et al. (1995) J. Neurotrauma. 12:53-63.

2) Robinson, A.P. et al. (1986) Immunology. 57:239-247.

3) Whiteland J.L. et al. (1995) J. Histochem and Cytochem. 43:313-320.

4) Issekutz, A. C. and T.B. Issekutz, (1992) Immunology. 76:655-661.

5) Yamazaki, T. et al. (1993) Am. J. of Pathology. 143:410-418.

6) Tamatani, T. et al. (1991) Eur. J. Immunol. 21:627-633.

7) Joan Y. et al. (2009) Am J Physiol Regul Integr Comp Physiol. 297:R1532-R1545.

8) Zheng Gen Piao et al. (2005) Pain. 121:219-231.
9) Abbadie C. et al. (2003) PNAS. 100:7947-7952.
10) Lee. et al. (2010) Molecular Pain. 6:79.
11) Summy-Long, J.Y. et al. (2008) Journal of Neuroendocrinology. 20:1224-1232.