Cedarlane › CL8943AP-3

Anti-Mouse Mac-3, Purified (Clone M3/84) (rat IgG1)

$613.00 CDN

Description

Cedarlane’s anti-mouse MAC-3 monoclonal antibody recognizes the MAC-3 antigen which is found on macrophages and some nonlymphoid tissues, but not on lymphocytes. It is a glycoprotein showing a broad band in SDS-PAGE, and is synthesized by macrophages (average molecular weight 92-110,000 daltons, depending on the origin of macrophages).
It reacts with peritoneal exudate macrophages where the exudate is provoked by thioglycollate, protease peptone, L. monocytogenes, lipopolysaccharide and concanavalin A. It does not react with thymocytes, spleen, lymph node or bone marrow cells in immunofluorescent flow cytometry.
MAC-3 is a general marker for macrophages and can be used to distinguish these cells from lymphocytes. Its Mw and cell distribution differentiate MAC-3 from MAC-2 and MAC-1 antigens.
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Flow Cytometry Analysis
Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages were stained with anti-Mac-3 (clone: M3/84) (filled histogram) or rat IgG1 isotype control (open histogram).

References
1) Springer, T.A. (1981). Monoclonal Antibody Analysis of Complex Biological Systems. J. Bio/. Chem. 256:3833-3839.
2) Knisley, K.A. and Weitlauf, H.M. (1993). Compartmentalized reactivity of M3/38 (anti-Mac-2) and M3/84 (anti-Mac-3) in the uterus of pregnant mice. Journal of Reproduction and Fertility. 97:521-527
3) Ho, M.K. and Springer, T.A. (1983). Tissue distribution, Structural Characterization, and Biosynthesis of Mac-3, a Macrophage Surface Glycoprotein Exhibiting Molecular Weight Heterogeneity. Journal of Biological Chemistry. 258:636-642.

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